| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,380,341 | 0 | G | A | 57.1% | ‑4.9 / 22.2 | 21 | intergenic (+31/‑72) | yfbL/yfbM | M28 family metallopeptidase/YfbM family protein |
| Reads supporting (aligned to +/- strand): ref base G (0/9); new base A (12/0); total (12/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.40e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.41e-05 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAA > NZ_CP009273/2380260‑2380419 | aTGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGc < 1:280812/90‑1 (MQ=255) gTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAAAGACATGCAC‑attttttttt > 1:37148/1‑90 (MQ=255) gTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAGGACAAGCAC‑attttatttt > 1:313083/1‑90 (MQ=255) aTGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTGCAGAAAAAACGGCAAACAC‑AATTTATTTTTCCt > 1:848259/1‑90 (MQ=255) ggTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGAGAAGCACAATTTTTTTTTcccca > 1:564174/1‑88 (MQ=255) gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACAAGGAC‑AATTTTTTTTTCCTCAt > 1:505132/1‑90 (MQ=255) tgtTATAACTTTGTTATACAACAGTAATTAATAAAAATTTCTCGAGGCGGTTCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCTCATc < 1:760240/90‑1 (MQ=255) tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCTCATc < 1:319467/90‑1 (MQ=255) tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCTCATc < 1:851258/90‑1 (MQ=255) tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCTCATc < 1:4056/90‑1 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCAC‑att < 2:89043/66‑1 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGACATGCAC‑att > 1:89043/1‑66 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCAAAAAAAAGAAAAAGAC‑AATTTTTTTTTCCTCCTCtttttttaa > 2:797635/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGAAAAACAA‑ATTTTTTTTTTTCTCATCTGtttttta > 2:760240/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAAAAAAGAAC‑AATTTTTTTTTTCTCATCttttttttt > 2:233268/1‑87 (MQ=255) caacaGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACAAGCAC‑ATTTTTTTTTTCCTCCTCTTTTTTTAGATGGGaaaa > 1:710258/1‑89 (MQ=255) ataaAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCAC‑ATTTTATTTTccc < 2:335051/55‑1 (MQ=255) ataaAAATTTCTCGAGGCGGGTCCAGAAAAAACGACATGCAC‑ATTTTATTTTccc > 1:335051/1‑55 (MQ=255) aaaaTTTCTCGAGGCGGGGCCCGAAAAAAAGACAAGCAC‑AATTTTTTTTTTCTCCTCTTTTTTTAAATGGGAAAAAAAAAAATCaaaaga > 2:354854/1‑86 (MQ=255) ttCTCGAGGCGGGTCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGaaa < 2:913955/90‑1 (MQ=255) gTCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGaa < 1:797635/90‑1 (MQ=255) | ATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAA > NZ_CP009273/2380260‑2380419 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |