Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,934,547	T→C	intergenic (‑97/+23)	lpxM ← / ← mepM	lauroyl‑Kdo(2)‑lipid IV(A) myristoyltransferase/murein DD‑endopeptidase MepM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,934,547	0	T	C	100.0%	106.9 / NA	31	intergenic (‑97/+23)	lpxM/mepM	lauroyl‑Kdo(2)‑lipid IV(A) myristoyltransferase/murein DD‑endopeptidase MepM
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/16);  total (15/16)

CGGATAAGGCAAAAATCAATCTGGTGATAGTGTAGCGGCGCAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGTACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGCGGCACAATCTCTTTGGCCTGCGCCAGGAATTCGCGAC  >  NZ_CP009273/1934464‑1934625                                                                                    |                                                                               cGGATAAGGCAAAAATCAATCTGGTGATAGTGTAGCGGCGCAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGc                                                                          <  1:824295/90‑1 (MQ=255)        ggCAAAAATCAATCTGGTGATACTGTAGCGGCGCAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAAt                                                                   <  1:856055/90‑1 (MQ=255)             aaaTCAATCTGGTGATAGTGTAGCGGCGCAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATg                                                              <  1:644842/90‑1 (MQ=255)             aaaTCAATCTGGTGATAGTGTAGCGGCGCAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATg                                                              <  2:446936/90‑1 (MQ=255)                 caatcTGGTGATAGTGTAGCGGCGCAACTTGCCCCGCCCCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGttaa                                                          <  2:35731/90‑1 (MQ=255)                 caatcTGGTGATAGTGTAGCGGCGCAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGttaa                                                          <  2:181837/90‑1 (MQ=255)                  aatcTGGTGATAGTGTAGCGGCGCAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGttaat                                                         >  1:157220/1‑90 (MQ=255)                   atcTGGTGATAGTGTAGCGGCGCAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGttaatt                                                        <  1:246233/90‑1 (MQ=255)                       ggTGATAGTGTAGCGGCGCAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATc                                                    <  1:541779/90‑1 (MQ=255)                            tAGTGTAGCGGCGCAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAAcc                                               >  2:236322/1‑90 (MQ=255)                               tgtAGCGGCGCAACTTGCCCCGCACCAAATAAAAAAAGCCTGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTa                                            <  2:414097/90‑1 (MQ=255)                                gtAGCGGCGCAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAg                                           >  2:540260/1‑90 (MQ=255)                                   gcggcgCAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATg                                                              <  1:149113/68‑1 (MQ=255)                                   gcggcgCAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATg                                                              >  2:149113/1‑68 (MQ=255)                                   gcggcgCAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTg                                        >  2:316153/1‑90 (MQ=255)                                    cggcgCAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGc                                       <  1:21958/90‑1 (MQ=255)                                     ggcgCAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGCg                                      >  1:183617/1‑90 (MQ=255)                                        gcAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGCGGca                                   <  1:98026/90‑1 (MQ=255)                                        gcAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGCGGca                                   >  1:739216/1‑90 (MQ=255)                                        gcAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGCGGca                                   >  1:611841/1‑90 (MQ=255)                                        gcAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACAGTAGCTGCGGca                                   >  2:102305/1‑90 (MQ=255)                                        gcAACTTGCCACGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGCGGca                                   >  1:571582/1‑90 (MQ=255)                                            cTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGCGGCACAAt                               <  2:277883/90‑1 (MQ=255)                                                ccccGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGCGGCACAATctct                           >  2:244708/1‑90 (MQ=255)                                                ccccGCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGCGGCACAATctct                           >  1:570383/1‑90 (MQ=255)                                                    gCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGCGGCACAATCTCTTTgg                       >  2:438952/1‑90 (MQ=255)                                                    gCACCAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGCGGCACAATCTCTTTgg                       >  2:559584/1‑90 (MQ=255)                                                       ccAAATAAAAAAAGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGCGGCACAATCTCTTTGGCCt                    <  1:818458/90‑1 (MQ=255)                                                                   aGCCGGTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGCGGCACAATCTCTTTGGCCTGCGCCAGGAAtt        >  2:463712/1‑90 (MQ=255)                                                                        gTACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGCGGCACAATCTCTTTGGCCTGCGCCAGGAATTCGcga   <  2:852032/90‑1 (MQ=255)                                                                         tACTGACTGCGCACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGCGGCACAATCTCTTTGGCCTGCGCCAGGAATTCGcgac  <  1:249451/90‑1 (MQ=255)                                                                                    |                                                                               CGGATAAGGCAAAAATCAATCTGGTGATAGTGTAGCGGCGCAACTTGCCCCGCACCAAATAAAAAAAGCCGGTACTGACTGCGTACCGGCTGCGAATGGATGTTAATTAATCAAACCGTAGCTGCGGCACAATCTCTTTGGCCTGCGCCAGGAATTCGCGAC  >  NZ_CP009273/1934464‑1934625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑