| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,122,248 | C→A | R41L (CGC→CTC) | wcaF ← | colanic acid biosynthesis acetyltransferase WcaF |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,122,248 | 0 | C | A | 93.3% | 42.6 / ‑2.6 | 15 | R41L (CGC→CTC) | wcaF | colanic acid biosynthesis acetyltransferase WcaF |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (3/11); total (4/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.00e-01 | |||||||||||
GGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCC > NZ_CP009273/2122169‑2122328 | ggTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGAGATACAATAc > 2:689878/1‑90 (MQ=255) cTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGAGATACAATACTTGTGGCGAcc < 2:774041/90‑1 (MQ=255) cTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGAGATACAATACTTGTGGCAAcc < 2:405523/90‑1 (MQ=255) acggacgAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGAGATACAATACTTGTGGCGACCAgg > 1:18213/1‑90 (MQ=255) tAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGAGATACAATACTTGTGGCGACCAGGCAAATAttg < 1:371686/90‑1 (MQ=255) tCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGAGATACAATACTTGTGGCGACCAGGCAAATATTGTTGCCTGTACTGc < 1:853890/90‑1 (MQ=255) cTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGAGATACAATCCTTGTGGCGACCAGGCAAATATTGTTGCCTGTACTGccc < 1:281143/90‑1 (MQ=255) cTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGAGATACAATACTTGTGGCGACCAGGCAAATATTGTTGCCTGTACTGccc < 1:494908/90‑1 (MQ=255) cTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGAGATACAATACTTGTGGCGACCAGGCAAATATTGTTGCCTGTACTGccc < 1:63356/90‑1 (MQ=255) tATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGAGATACAATACTTGTGGCGACCAGGCAAATATTGTTGCCTGTACTGCcca > 2:477215/1‑90 (MQ=255) tttttGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGAGATACAATACTTGTGGCGACCAGGCAAATATTGTTGCCTGTACTGCccacc < 1:115048/90‑1 (MQ=255) ccGAATAAACGTAATAAAAAAGCCCGCCAGAGATACAATACTTGTGGCGACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGc < 1:467953/90‑1 (MQ=255) ccGAATAAACGTAATAAAAAAGCCCGCCAGAGATACAATACTTGTGGCGACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGc < 2:469439/90‑1 (MQ=255) aaaaGCCCGCCAGAGATACAATACTTGTGGCGACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTgcc < 1:905492/90‑1 (MQ=255) gCCCGCCAGCGATACAATACTTGTGGCGACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGccc > 1:836103/1‑90 (MQ=255) | GGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCC > NZ_CP009273/2122169‑2122328 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |