Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 2,122,528 T→G T202P (ACC→CCC)  wcaE ← colanic acid biosynthesis glycosyltransferase WcaE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,122,5280TG86.4% 63.5 / 3.1 22T202P (ACC→CCC) wcaEcolanic acid biosynthesis glycosyltransferase WcaE
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base G (11/8);  total (12/10)
Fisher's exact test for biased strand distribution p-value = 5.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

ATTCAGCCCAAAAGCCAGGCACATGTAATATTTGTCGTTGGACTTTTTTCGCGTCAGCACACAATTCCATATTATTGGTGGTAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTAAATGCATAACCGGCTTTATACATTTTGGCTGCCAG  >  NZ_CP009273/2122447‑2122612
                                                                                 |                                                                                    
aTTCAGCCCAAAAGCCAGGCACATGTAATATTTGTCGTTGGACTTTTTTCGCGTCAGCACACAATTCCATATTATTGGTGGTAGATAccc                                                                              <  2:196455/90‑1 (MQ=255)
      cccAAAAGCCAGGCACATGTAATATTTGTCGTTGGACTTTTTTCGCGTCAGCACACAATTCCATATTATTGGTGGTAGATACcccaccca                                                                        >  1:166501/1‑90 (MQ=255)
           aaGCCAGGCACATGTAATATTTGTCGTTGGACTTTTTTCGCGTCAGCACACAATTCCATATTATTGGTGGGAGATACCCCACCCATGGaa                                                                   <  2:33594/90‑1 (MQ=255)
           aaGCCAGGCACATGTAATATTTGTCGTTGGACTTTTTTCGCGTCAGCACACAATTCCATATTATTGGTGGGAGATACCCCACCCATGGaa                                                                   <  2:151423/90‑1 (MQ=255)
                  gCACATGTAATATTTGTCGTTGGACTTTTTTCGCGTCAGCACACAATTCCATATTATTGGTGGGAGATACCCCACCCATGGAAAATTCAg                                                            <  1:781186/90‑1 (MQ=255)
                  gCACATGTAATATTTGTCGTTGGACTTTTTTCGCGTCAGCACACAATTCCATATTATTGGTGGGAGATACCCCACCCATGGAAAATTCAg                                                            <  2:274036/90‑1 (MQ=255)
                      aTGTAATATTTGTCGTTGGACTTTTTTCGCGTCAGCACACAATTCCATATTATTGGTGGGAGATACCCCACCCATGGAAAATTCAGacac                                                        >  1:321504/1‑90 (MQ=255)
                               ttGTCGTTGGACTTTTTTCGCGTCAGCACACAATTCCATATTATTGTTGGGAGAAACCCCACCCATGGAAAATTCAGACACCAGGCCAtt                                               >  1:281757/1‑90 (MQ=255)
                               ttGTCGTTGGACTTTTTTCGCGTCAGCACACAATTCCATATTATTGGTGGGAGATACCCCACCCATGGAAAATGCAGACACCAGGCCAtt                                               >  2:639562/1‑90 (MQ=255)
                                 gTCGTTGGACTTTTTTCGCGTCAGCACACAATTCCATATTATTGGTGGGAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGa                                             <  1:35491/90‑1 (MQ=255)
                                       ggACTTTTTTCGCGTCAGCACACAATTCCATATTATTGGTGGGAg                                                                                    <  2:734771/45‑1 (MQ=255)
                                             ttttCGCGTCAGCACACAATTCCATATTATTGGTGGTAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTAAATg                                 <  2:166501/90‑1 (MQ=255)
                                                    gTCAGCACACAATTCCATATTATTGGTGGGAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTAAATGCATAAcc                          >  1:685428/1‑90 (MQ=255)
                                                     tCAGCACACAATTCCATATTATTGGTGGGAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTAAATGCATAACCg                         <  1:647694/90‑1 (MQ=255)
                                                             caATTCCATATTATTGGTGGGAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTAAATGCATAACCGGCTTtata                 >  2:69420/1‑90 (MQ=255)
                                                                  ccATATTATTGGTGGGAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTAAATGCATAACCGGCTTTATACAttt            >  1:120933/1‑90 (MQ=255)
                                                                       ttattGGTGGGAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTAAATGCATAACCGGCTTTATACATTTTGGCt       >  2:882998/1‑90 (MQ=255)
                                                                       ttattGGTGGGAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTAAATGCATAACCGGCTTTATACATTTTGGCt       >  1:576710/1‑90 (MQ=255)
                                                                         attGGTGGGAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTa                                     <  2:448933/58‑1 (MQ=255)
                                                                         attGGTGGGAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTa                                     >  1:448933/1‑58 (MQ=255)
                                                                         attGGTGGGAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTAAATGCATAACCGGCTTTATACATTTTGGCTGc     >  1:304755/1‑90 (MQ=255)
                                                                         attGGTGGGAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTAAATGCATAACCGGCTTTATACATTTTGGCTGc     >  1:892388/1‑90 (MQ=255)
                                                                            ggtgggAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTAAATGCATAACCGGCTTTATACATTTTGGCTGCCAg  >  1:392863/1‑90 (MQ=255)
                                                                                 |                                                                                    
ATTCAGCCCAAAAGCCAGGCACATGTAATATTTGTCGTTGGACTTTTTTCGCGTCAGCACACAATTCCATATTATTGGTGGTAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTAAATGCATAACCGGCTTTATACATTTTGGCTGCCAG  >  NZ_CP009273/2122447‑2122612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑