Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,122,677	A→G	F152S (TTC→TCC)	wcaE ←	colanic acid biosynthesis glycosyltransferase WcaE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,122,677	0	A	G	100.0%	95.9 / NA	28	F152S (TTC→TCC)	wcaE	colanic acid biosynthesis glycosyltransferase WcaE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/16);  total (12/16)

TATACATTTTGGCTGCCAGCGCGTAGTCGGAAGAAACTTTATATTCCAGGTCATAACGCCATTTTTTCAAGCCGGATACAGGGAAAAATATCGCCTGATGACTGGCGGGCAGGCTGTGATAAATATACCAGCCCGGTTTGGCGCTACGTTTAATTTTATGCCCGTCGCC  >  NZ_CP009273/2122594‑2122762                                                                                    |                                                                                      tataCATTTTGGCTGCCAGCGCGTAGTCGGAAGAAACTTTATATTCCAGGTCATAACGCCATTTTTTCAAGCCGGATACAGGGGAAAata                                                                                 >  2:757802/1‑90 (MQ=255)   taCATTTTGGCTGCCAGCGCGTAGTCGGAAGAAACTTTATATTCCAGGTCATAACGCCATTTTTTCAAGCCGGATACAGGGGAAAATATc                                                                               <  2:281757/90‑1 (MQ=255)      aTTTTGGCTGCCAGCGCGTAGTCGGAAGAAACTTTATATTCCAGGTCATAACGCCATTTTTTCAAGCCGGATACAGGGGAAAATATCGcc                                                                            <  1:757802/90‑1 (MQ=255)       ttttGGCTGCCAGCGCGTAGTCGGAAGAAACTTTATATTCCAGGTCATAACGCCATTTTTTCAAGCCGGATACAGGGGAAAATATCGCCt                                                                           >  1:135547/1‑90 (MQ=255)       ttttGGCTGCCAGCGCGTAGTCGGAAGAAACTTTATATTCCAGGTCATAACGCCATTTTTTCAAGCCGGATACAGGGGAAAATATCGCCt                                                                           >  2:89529/1‑90 (MQ=255)       ttttGGCTGCCAGCGCGTAGTCGGAAGAAACTTTATATTCCAGGTCATAACGCCATTTTTTCAAGCCGGATACAGGGGAAAATATCGCCt                                                                           >  1:239774/1‑90 (MQ=255)       ttttGGCTGCCAGCGCGTAGTCGGAAGAAACTTTATATTCCAGGTCATAACGCCATTTTTTCAAGCCGGATACAGGGGAAAATATCGCCt                                                                           >  1:340799/1‑90 (MQ=255)           ggCTGCCAGCGCGTAGTCGGAAGAAACTTTATATTCCAGGTCATAACGCCATTTTTTCAAGCCGGATACAGGGGAAAATATCGCCtgatg                                                                       <  1:95695/90‑1 (MQ=255)                 cAGCGCGTAGTCGGAAGAAACTTTATATTCCAGGTCATAACGCCATTTTTTCAAGCCGGATACAGGGGAAAATATCGCCTGATGACTGGc                                                                 >  1:138785/1‑90 (MQ=255)                     gcgTAGTCGGAAGAAACTTTATATTCCAGGTCATAACGCCATTTTTTCAAGCCGGATACAGGGGAAAATATCGCCTGATGACTGGCGGGc                                                             <  2:784036/90‑1 (MQ=255)                         aGTCGGAAGAAACTTTATATTCCAGGTCATAACGCCATTTTTTCAAGCCGGATACAGGGGAAAATATCGCCTGATGACTGGCGGGCAGGc                                                         <  1:214890/90‑1 (MQ=255)                         aGTCGGAAGAAACTTTATATTCCAGGTCATAACGCCATTTTTTCAAGCCGGATACAGGGGAAAATATCGCCTGATGACTGGCGGGCAGGc                                                         <  1:689878/90‑1 (MQ=255)                         aGTCGGAAGAAACTTTATATTCCAGGTCATAACGCCATTTTTTCAAGCCGGATACAGGGGAAAATATCGCCTGATGACTGGCGGGCAGGc                                                         <  1:281649/90‑1 (MQ=255)                         aGTCGGAAGAAACTTTATATTCCAGGTCATAACGCCATTTTTTCAAGCCGGATACAGGGGAAAATATCGCCTGATGACTGGCGGGCAGGc                                                         <  2:135547/90‑1 (MQ=255)                           tCGGAAGAAACTTTATATTCCAGGTCATAACGCCATTTTTTCAAGCCGGATACAGGGGAAAATATCGCCTGATGACTGGCGGGCAGGCtg                                                       <  2:892708/90‑1 (MQ=255)                            cGGAAGAAACTTTATATTCCAGGTCATAACGCCATTTTTTCAAGCCGGATACAGGGGAAAATATCGCCTGATGACTGGCGGGCAGGCtgt                                                      >  1:561091/1‑90 (MQ=255)                              gaagaaACTTTATATTCCAGGTCATAACGCCATTTTTTCAAGCCGGATACAGGGGAAAATATCGCCTGATGACTGGCGGGCAGGCTGTGa                                                    <  1:477215/90‑1 (MQ=255)                              gaagaaACTTTATATTCCAGGTCATAACGCCATTTTTTCAAGCCGGATACAGGGGAAAATATCGCCTGATGACTGGCGGGCAGGCTGTGa                                                    <  1:882998/90‑1 (MQ=255)                              gaagaaACTTTATATTCCAGGTCATAACGCCATTTTTTCAAGCCGGATACAGGGGAAAATATCGCCTGATGACTGGCGGGCAGGCTGTGa                                                    <  2:428704/90‑1 (MQ=255)                                    aCTTTATATTCCAGGTCATAACGCCATTTTTTCAAGCCGGATACAGGGGAAAATATCGCCTGATGACTGGCGGGGAGGCTTGGATAAata                                              >  1:265237/1‑90 (MQ=255)                                            ttCCAGGTCATAACGCCATTTTTTCAAGCCGGATACAGGGGAAAATATCGCCTGATGACTGGCGGGc                                                             <  2:252266/67‑1 (MQ=255)                                            ttCCAGGTCATAACGCCATTTTTTCAAGCCGGATACAGGGGAAAATATCGCCTGATGACTGGCGGGc                                                             >  1:252266/1‑67 (MQ=255)                                             tCCAGGTCATAACGCCATTTTTTCAAGCCGGATACAGGGGAAAATATCGCCTGATGACTGGCGGGCAGGCTGTGATAAATATACCAGccc                                     <  2:576710/90‑1 (MQ=255)                                               cAGGTCATAACGCCATTTTTTCAAGCCGGATACAGGGGAAAATATCGCCTGATGACTGGCGGGCCGGGTGTGGTAAATATACCAGCCCgg                                   >  1:685713/1‑90 (MQ=255)                                               cAGGTCATAACGCCATTTTTTCAAGCCGGATACAGGGGAAAATATCGCCTGATGACTGGCGGGCAGGCTGTGATAAATATACCAGCCCgg                                   >  2:664621/1‑90 (MQ=255)                                                 ggTCATAACGCCATTTTTTCAAGCCGGATACAGGGGAAAATATCGCCTGATGACTGGCGGGCAGGCTGTGATAAATATAACAGCCCGGtt                                 >  1:755028/1‑90 (MQ=255)                                                         cGCCATTTTTTCAAGCCGGATACAGGGGAAAATATCGCCTGATGACTGGCGGGCAGGCTGTGATAAATATACCAGCCCGGTTTGGCGCTa                         <  2:213869/90‑1 (MQ=255)                                                                                aGGGGAAAATATCGCCTGATGACTGGCGGGCAGGCTGTGATAAATATACCAGCCCGGTTTGGCGCTACGTTTAATTTTATGCCCGTCGcc  <  2:392863/90‑1 (MQ=255)                                                                                    |                                                                                      TATACATTTTGGCTGCCAGCGCGTAGTCGGAAGAAACTTTATATTCCAGGTCATAACGCCATTTTTTCAAGCCGGATACAGGGAAAAATATCGCCTGATGACTGGCGGGCAGGCTGTGATAAATATACCAGCCCGGTTTGGCGCTACGTTTAATTTTATGCCCGTCGCC  >  NZ_CP009273/2122594‑2122762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑