Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,229,956	A→G	K405E (AAA→GAA)	preA →	NAD‑dependent dihydropyrimidine dehydrogenase subunit PreA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,229,956	0	A	G	100.0%	65.8 / NA	19	K405E (AAA→GAA)	preA	NAD‑dependent dihydropyrimidine dehydrogenase subunit PreA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/7);  total (12/7)

GAGAAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACACCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGA  >  NZ_CP009273/2229869‑2230028                                                                                        |                                                                         gagaTATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGaggaa                                                                        >  2:363554/1‑90 (MQ=255) gagaAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGaggaa                                                                        >  1:239671/1‑90 (MQ=255) gagaAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGaggaa                                                                        >  1:25183/1‑90 (MQ=255) gagaAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGaggaa                                                                        >  1:847920/1‑90 (MQ=255) gagaAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGGGAAGTTTAAGAAAGGCGaggaa                                                                        >  2:618670/1‑90 (MQ=255)       tgtgtgGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGGAAGAacac                                                                  <  2:569837/90‑1 (MQ=255)            gggTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGGAAGAACACCCGGt                                                             >  1:417952/1‑90 (MQ=255)                tGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGGAAGAACACCCGGTAACg                                                         <  1:372596/90‑1 (MQ=255)                  tCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGGAAGAACACCCGGTAACGtt                                                       >  1:639323/1‑90 (MQ=255)                       tttGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGGAAGAACACCCGGTAACg                                                         <  2:642412/83‑1 (MQ=255)                       tttGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGGAAGAACACCCGGTAACg                                                         >  1:642412/1‑83 (MQ=255)                               cACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGGAAGAACACCCGGTAACGTTGTAAAACCAgaga                                          >  2:851817/1‑90 (MQ=255)                                       cccGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGGAAGAACACCCGGTAACGTTGTAAAACCAGAGACGCATCCg                                  <  1:76162/90‑1 (MQ=255)                                        ccGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGGAAGAACACCCGGTAACGTTGTAAAACCAGAGACGCATCCgg                                 <  1:557756/90‑1 (MQ=255)                                                       gAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGGAAGAacac                                                                  <  1:234787/42‑1 (MQ=255)                                                       gAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGGAAGAacac                                                                  >  2:234787/1‑42 (MQ=255)                                                       gAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGGAAGAACACCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTc                  >  2:403934/1‑90 (MQ=255)                                                       gAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGGAAGAACACCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTc                  >  1:53111/1‑90 (MQ=255)                                                         gCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGGAAGAACACCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGc                >  1:765810/1‑90 (MQ=255)                                                         gCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGGAAGAACACCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGc                >  1:129833/1‑90 (MQ=255)                                                            cGGGGAAGTGAAGTTTAAGAAAGGCGAGGAAGAACACCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCgg             >  2:337062/1‑90 (MQ=255)                                                              gggAAGTGAAGTTTAAGAAAGGCGAGGAAGAACACCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGAt           >  2:667236/1‑90 (MQ=255)                                                                   gtgaagtTTAAGAAAGGCGAGGAAGAACACCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGc      >  2:513954/1‑90 (MQ=255)                                                                       agtTTAAGAAAGGCGAGGAAGAACACCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGa  <  1:553273/90‑1 (MQ=255)                                                                                        |                                                                         GAGAAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTTAAGAAAGGCGAGAAAGAACACCCGGTAACGTTGTAAAACCAGAGACGCATCCGGCATTTGGTTCCTGTCGCCGGATGCGGCTTGA  >  NZ_CP009273/2229869‑2230028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑