| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,328,974 | G→C | P384A (CCA→GCA) | yfaA ← | DUF2138 domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,328,974 | 0 | G | C | 90.5% | 61.3 / 0.4 | 21 | P384A (CCA→GCA) | yfaA | DUF2138 domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base G (2/0); new base C (12/7); total (14/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.55e-01 | |||||||||||
GAGTCTGGCTTACCGGCAAAACACCTTCTGGCGCTTTGCTTTCGTGCGCACCAATATTTTGCGTAAACAGTTTCCCTGGCAATTGCGCCTGTTCGGCAGTGCCATCAAACTGACCGACAAACAGCGGGGTTTGCAATTTTGAGTCTTC > NZ_CP009273/2328896‑2329043 | gAGTCTGGCTTACCGGCAAAACACCTTCTGGCGCTTTGCTTTCGTGCGCACCAATATTTTGCGTAAACAGTTTCCCTGCCAATTGCGCCt > 2:840727/1‑90 (MQ=255)gAGTCTGGCTTACCGGCAAAACACCTTCTGGCGCTTTGCTTTCGTGCGCACCAATATTTTGCGTAAACAGTTTCCCTGCCAATTGCGCCt > 2:751619/1‑90 (MQ=255) gCTTACCGGCAAAACACCTTCTGGCGCTTTGCTTTCGTGCGCACCAATATTTTGCGTAAACAGTTTCCCTGCCAATTGCGCCTGTTCGGc > 1:34786/1‑90 (MQ=255) ccGGCAAAACACCTTCTGGCGCTTTGCTTTCGTGCGCACCAATATTTTGCGTAAACAGTTTCCCTGCCAATTGCGCCTGTTCGGCAGTGc > 2:101727/1‑90 (MQ=255) acCTTCTGGCGCTTTGCTTTCGTGCGCACCAATATTTTGCGTAAACAGTTTCCCTGCCAATTGCGCCTGTTCGGCAGTGCCATCAAACTg < 1:632787/90‑1 (MQ=255) ccTTCTGGCGCTTTGCTTTCGTGCGCACCAATATTTTGCGTAAACAGTTTCCCTGCCAATTGCGCCTGTTCGGCAGTGCCATCAAACTGa > 2:637698/1‑90 (MQ=255) cTTCTGGCGCTTTGCTTTCGTGCGCACCAATATTTTGCGTAAACAGTTTCCCTGCCAATTGCGCCTGTTCGGCAGTGCCATCAAACTGAc > 1:301434/1‑90 (MQ=255) cTTCTGGCGCTTTGCTTTCGTGCGCACCAATATTTTGCGTAAACAGTTTCCCTGCCAATTGCGCCTGTTCGGCAGTGCCATCAAACTGAc > 2:309961/1‑90 (MQ=255) ttCTGGCGCTTTGCTTTCGTGCGCACCAATATTTTGCGTAAACAGTTTCCCTGCCAATTGCGCCTGTTCGGCAGTGCCATCAAACTGAcc > 1:533226/1‑90 (MQ=255) gctttCGTGCGCACCAATATTTTGCGTAAACAGTTTCCCTGCCAATTGCGCCTGTTCGGCAGTGCCATCAAACTGACCGACAAACAGCgg < 1:639813/90‑1 (MQ=255) aaTATTTTGCGTAAACAGTTTCCCTGGCAATTGCGCCTGTTCGGCAGTGCCATCAAACTGACCGACAAACAGCGGGGTTTGCAATTTTGa > 1:519942/1‑90 (MQ=255) atatTTTGCGTAAACAGTTTCCCTGCCAATTGCGCCTGTTCGGCAGTGCCATCAAACTGACCGACAAACAGCGGGGTTTGCAATTTTGAg < 1:210291/90‑1 (MQ=255) atatTTTGCGTAAACAGTTTCCCTGCCAATTGCGCCTGTTCGGCAGTGCCATCAAACTGACCGACAAACAGCGGGGTTTGCAATTTTGAg < 2:34786/90‑1 (MQ=255) ttGCGTAAACCGTTTCCCTGCCAACTGCGCCTGTTCGGCAGTGCCATCAAACTGACCGACAAACAGCGGGGTTTGCAATTTTGAGTCTTc < 2:573535/90‑1 (MQ=255) ttGCGTAAACAGTTTCCCTGGCAATTGCGCCTGTTCGGCAGTGCCATCAAACTGACCGACAAACAGCGGGGTTTGCAATTTTGAGTCTTc > 1:733010/1‑90 (MQ=255) ttGCGTAAACAGTTTCCCTGCCAATTGCGCCTGTTCGGCAGTGCCATCAAACTGAcc > 2:11535/1‑57 (MQ=255) ttGCGTAAACAGTTTCCCTGCCAATTGCGCCTGTTCGGCAGTGCCATCAAACTGAcc < 1:11535/57‑1 (MQ=255) ttGCGTAAACAGTTTCCCTGCCAATTGCGCCTGTTCGGCAGTGCCATCAAACTGACCGACAAACAGCGGGGTTTGCAATTTTGAGTCTTc < 1:98565/90‑1 (MQ=255) ttGCGTAAACAGTTTCCCTGCCAATTGCGCCTGTTCGGCAGTGCCATCAAACTGACCGACAAACAGCGGGGTTTGCAATTTTGAGTCTTc > 1:557725/1‑90 (MQ=255) ttGCGTAAACAGTTTCCCTGCCAATTGCGCCTGTTCGGCAGTGCCATCAAACTGACCGACAAACAGCGGGGTTTGCAATTTTGAGTCTTc > 2:826206/1‑90 (MQ=255) ttGCGTAAACAGTTTCCCTGCCAATTGCGCCTGTTCGGCAGGGCCATCAAACTGACCGACAAACAGCGGGGTTTGCAATTTTGAGGCTTc > 1:338185/1‑90 (MQ=255) | GAGTCTGGCTTACCGGCAAAACACCTTCTGGCGCTTTGCTTTCGTGCGCACCAATATTTTGCGTAAACAGTTTCCCTGGCAATTGCGCCTGTTCGGCAGTGCCATCAAACTGACCGACAAACAGCGGGGTTTGCAATTTTGAGTCTTC > NZ_CP009273/2328896‑2329043 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |