Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,345,414	T→G	H147P (CAC→CCC)	glpT ←	glycerol‑3‑phosphate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,345,414	0	T	G	100.0%	103.0 / NA	29	H147P (CAC→CCC)	glpT	glycerol‑3‑phosphate transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (13/16);  total (13/16)

AACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATC  >  NZ_CP009273/2345327‑2345488                                                                                        |                                                                           aaCAGCAGCGGCGGAATACCCCCCCCGACGTTGTGCGCCCAGTTCCACCCTGACCCAATGCCCCCACGTTCTTTCTGCGACCACCAGGGc                                                                          <  2:300346/90‑1 (MQ=255) aaCAGCAGCGGCGGAATACCACCACCGCCGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGc                                                                          <  2:37408/90‑1 (MQ=255) aaCAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGc                                                                          <  2:154609/90‑1 (MQ=255)  aCAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCa                                                                         <  1:84690/90‑1 (MQ=255)    agcagcGGCGGAATACCACCCCCGACGTTGTGCGCACAGTTCCACACGGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCAcc                                                                       <  2:495542/90‑1 (MQ=255)          ggcggAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTa                                                                 >  1:177504/1‑90 (MQ=255)           gcggAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTAc                                                                <  2:813272/90‑1 (MQ=255)                       caccGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACAcggcg                                                    >  1:318537/1‑90 (MQ=255)                       caccGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACAcggcg                                                    >  1:556852/1‑90 (MQ=255)                        accGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGc                                                                          >  2:45359/1‑67 (MQ=255)                        accGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGc                                                                          <  1:45359/67‑1 (MQ=255)                                 gtgCGCACAGTTCCACCCTGACACAATGCCGCCACGTTCTTTCTGCAACCACCAGGGCACCATAGTTCGCCCACACGGCGGCCACCCCAt                                          <  2:901918/90‑1 (MQ=255)                                        caGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCCCCATAGTACGACCACACGGCGGCCACCCCATCCCCTgg                                   <  1:838811/90‑1 (MQ=255)                                          gTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGaa                                 >  1:126581/1‑90 (MQ=255)                                                  cTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGc                                                                          <  2:665376/41‑1 (MQ=255)                                                  cTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGc                         >  2:748756/1‑90 (MQ=255)                                                    gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATcccc                                      <  2:221306/75‑1 (MQ=255)                                                    gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATcccc                                      >  1:221306/1‑75 (MQ=255)                                                    gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCag                       >  2:563512/1‑90 (MQ=255)                                                     acacAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCaga                      <  2:177504/90‑1 (MQ=255)                                                        caATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGa                   <  1:660858/90‑1 (MQ=255)                                                          aTGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGaac                 >  2:706034/1‑90 (MQ=255)                                                          aTGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGaac                 >  1:162166/1‑90 (MQ=255)                                                                ccACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGt           >  1:109180/1‑90 (MQ=255)                                                                  aCGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGa                   <  1:190943/80‑1 (MQ=255)                                                                  aCGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGa                   >  2:190943/1‑80 (MQ=255)                                                                         ttctGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATc  <  1:386283/90‑1 (MQ=255)                                                                           ctGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCAt                                          <  1:219822/48‑1 (MQ=255)                                                                           ctGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCAt                                          >  2:219822/1‑48 (MQ=255)                                                                                        |                                                                           AACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATC  >  NZ_CP009273/2345327‑2345488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑