Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,410,868	A→T	G103G (GGA→GGT)	yfcC →	putative basic amino acid antiporter YfcC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,410,868	0	A	T	100.0%	124.6 / NA	33	G103G (GGA→GGT)	yfcC	putative basic amino acid antiporter YfcC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (14/19);  total (14/19)

ACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCA  >  NZ_CP009273/2410781‑2410953                                                                                        |                                                                                      aCCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTc                                                                                     <  1:76144/90‑1 (MQ=255)    tGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGaa                                                                                  <  1:898272/90‑1 (MQ=255)           cACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACggg                                                                           >  2:170624/1‑90 (MQ=255)            aCCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGa                                                                          >  1:335929/1‑90 (MQ=255)            aCCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGa                                                                          >  1:817364/1‑90 (MQ=255)             ccGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGAc                                                                         <  1:79311/90‑1 (MQ=255)               gcgTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAg                                                                       >  1:94270/1‑90 (MQ=255)               gcgTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAg                                                                       >  1:367158/1‑90 (MQ=255)                   aCAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGt                                                                   <  2:581219/90‑1 (MQ=255)                    cAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGtt                                                                  >  1:101051/1‑90 (MQ=255)                       cTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGAc                                                                         >  2:828020/1‑80 (MQ=255)                       cTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGAc                                                                         <  1:828020/80‑1 (MQ=255)                        tGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGca                                                              <  2:415584/90‑1 (MQ=255)                        tGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGca                                                              <  2:817364/90‑1 (MQ=255)                         gTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGcat                                                             >  2:820643/1‑90 (MQ=255)                            cACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGcatcat                                                          <  2:333768/90‑1 (MQ=255)                             acgacgGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGcatcatc                                                         <  1:740604/90‑1 (MQ=255)                             acgacgGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGcatcatc                                                         <  1:349679/90‑1 (MQ=255)                             acgacgGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGcatcatc                                                         <  1:30223/90‑1 (MQ=255)                                        tGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCt                                              >  2:637977/1‑90 (MQ=255)                                                    ccTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTggcgg                                  <  1:873725/90‑1 (MQ=255)                                                      tgatgaACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGgcggcg                                >  1:30852/1‑90 (MQ=255)                                                        atgaACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCTGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGcgcg                              <  1:761588/90‑1 (MQ=255)                                                           aaCTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGttt                           <  2:94270/90‑1 (MQ=255)                                                            aCTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTg                          >  2:536918/1‑90 (MQ=255)                                                             cTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTgg                         <  1:211522/90‑1 (MQ=255)                                                              ttCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGc                        >  1:359361/1‑90 (MQ=255)                                                                       gAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATg               <  2:520999/90‑1 (MQ=255)                                                                               aaCCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAgg       >  1:716088/1‑90 (MQ=255)                                                                                    cAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTggcgg                                  >  1:798352/1‑58 (MQ=255)                                                                                    cAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTggcgg                                  <  2:798352/58‑1 (MQ=255)                                                                                    cAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCa  <  1:61573/90‑1 (MQ=255)                                                                                     aGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGa      <  2:177479/85‑1 (MQ=255)                                                                                        |                                                                                      ACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCA  >  NZ_CP009273/2410781‑2410953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑