| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,414,135 | T→C | G12G (GGT→GGC) | yfcG → | GSH‑dependent disulfide bond oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,414,135 | 0 | T | C | 97.2% | 121.6 / ‑3.8 | 36 | G12G (GGT→GGC) | yfcG | GSH‑dependent disulfide bond oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (19/16); total (19/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.97e-01 | |||||||||||
CCGCGCTTTCTATACTTGTGGTGTTCAGATGATTAAACAACCGGAGCTGCAATGATCGATCTCTATTTCGCCCCGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCA > NZ_CP009273/2414049‑2414212 | ccgcGCTTTCTATACTTGTGGTGTTCAGATGATTAAACAACCGGAGCTGCAATGATCGTTCTCTATTTCGCCCCGACACCCAATGGCcac < 1:528693/90‑1 (MQ=255) ttGTGGTGTTCAGATGATTAAACAACCGGAGCTGCAATGATCGATCTCTATTTCGCCCCGACACCCAATGGCcac < 2:22850/75‑1 (MQ=255) ttGTGGTGTTCAGATGATTAAACAACCGGAGCTGCAATGATCGATCTCTATTTCGCCCCGACACCCAATGGCcac > 1:22850/1‑75 (MQ=255) ttGTGGTGTTCAGATGATTAAACAACCGGAGCTGCAATGATCGATCTCTATTTCGCCCCGACACCCAATGGCCACAAAATTACGCTGttt > 2:851395/1‑90 (MQ=255) ttGTGGTGTTCAGATGATTAAACAACCGGAGCTGCAATGATCGATCTCTATTTCGCCCCGACACCCAATGGCCACAAAATTACGCTGttt > 1:343794/1‑90 (MQ=255) tgtgGTGTTCAGATGATTAAACAACCGGAGCTGCAATGATCGATCTCTATTTCGCCCCGACACCCAATGGCCACAAAATTACGCTGTTtc > 2:593501/1‑90 (MQ=255) gtggtgTTCAGATGATTAAACAACCGGAGCTGCAATGATCGATCTCTATTTCGCCCCGACACCCAATGGCCACAAAATTACGCTGTTtct > 1:902589/1‑90 (MQ=255) gtggtgTTCAGATGATTAAACAACCGGAGCTGCAATGATCGATCTCTATTTCGCCCCGACACCCAATGGCCACAAAATTACGCTGTTtct > 1:459040/1‑90 (MQ=255) gtggtgTTCAGATGATTAAACAACCGGAGCTGCAATGATCGATCTCTATTTCGCCCCGACACCCAATGGCCACAAAATTACGCTGTTtct > 1:430927/1‑90 (MQ=255) tgtTCAGATGATTAAACAACCGGAGCTGCAATGATCGATCTCTATTTCGCCCCGACACCCAATGGCCACAAAATTACGCTGTTTCTCgaa > 1:145013/1‑90 (MQ=255) gtTCAGATGATTAAACAACCGGAGCTGCAATGATCGATCTCTATTTCGCCCCGACACCCAATGGCCACAAAATTACGCTGTTTCTCgaag > 1:690710/1‑90 (MQ=255) aGATGATTAAACAACCGGAGCTGCAATGATCGATCTCTATTTCGCCCCGACACCCAATGGCCACAAAATTACGCTGTTTCTCGAAGAAGc < 1:306181/90‑1 (MQ=255) ttAAACAACCGGAGCTGCAATGATCGATCTCTATTTCGCCCCGACACCCAATGGCCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCt > 2:621832/1‑90 (MQ=255) ttAAACAACCGGAGCTGCAATGATCGATCTCTATTTCGCCCCGACACCCAATGGCCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCt > 1:228139/1‑90 (MQ=255) tAAACAACCGGAGCTGCAATGATCGATCTCTATTTCGCCCCGACACCCAATGGCCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTg < 2:690710/90‑1 (MQ=255) acCGGAGCTGCAATGATCGATCTCTATTTCGCCCCGACACCCAATGGCCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTAt > 2:65520/1‑90 (MQ=255) ggAGCTGCAATGATCGATCTCTATTTCGCCCCGACACCCAATGGCCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGc < 1:650327/90‑1 (MQ=255) ggAGCTGCAATGATCGATCTCTATTTCGCCCCGACACCCAATGGCCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGc < 1:770571/90‑1 (MQ=255) gAGCTGCAATGATCGATCTCTATTTCGCCCCGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCt < 1:348783/90‑1 (MQ=255) gAGCTGCAATGATCGATCTCTATTTCGCCCCGACACCCAATGGCCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCt < 1:180661/90‑1 (MQ=255) gCTGCAATGATCGATCTCTATTTCGCCCCGACACCCAATGGCCACAAAATTACGc > 1:705899/1‑55 (MQ=255) gCTGCAATGATCGATCTCTATTTCGCCCCGACACCCAATGGCCACAAAATTACGc < 2:705899/55‑1 (MQ=255) gCAATGATCGATCTCTATTTCGCCCCGACACCCAATGGCCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGAtt < 2:530505/90‑1 (MQ=255) aaTGATCGATCTCTATTTCGCCCCGACACCCAATGGCCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTaa < 1:477942/90‑1 (MQ=255) tGATCGATCTCTATTTCGCCCCGACACCCAATGGCCAC‑AAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGt < 1:581486/90‑1 (MQ=255) gatcgatcTCTATTTCGCCCCGACACCCAATGGCCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGt < 2:607401/90‑1 (MQ=255) atcgatcTCTATTTCGCCCCGACACCCAATGGCCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTa < 2:145013/90‑1 (MQ=255) cGCCCCGACACCCAATGGCCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAgg < 2:430927/90‑1 (MQ=255) cGCCCCGACACCCAATGGCCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAgg > 1:554538/1‑90 (MQ=255) gCCCCGACACCCAATGGCCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGAtt > 1:700612/1‑69 (MQ=255) gCCCCGACACCCAATGGCCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGAtt < 2:700612/69‑1 (MQ=255) gCCCCGACACCCAATGGCCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGc > 2:659911/1‑90 (MQ=255) ccGACACCCAATGGCCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGAtt > 2:273091/1‑66 (MQ=255) ccGACACCCAATGGCCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGAtt < 1:273091/66‑1 (MQ=255) gACACCCAATGGCCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCa > 2:13662/1‑90 (MQ=255) gACACCCAATGGCCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCa > 1:324187/1‑90 (MQ=255) | CCGCGCTTTCTATACTTGTGGTGTTCAGATGATTAAACAACCGGAGCTGCAATGATCGATCTCTATTTCGCCCCGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCA > NZ_CP009273/2414049‑2414212 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |