Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,536,106	T→C	E261G (GAA→GGA)	cysP ←	thiosulfate/sulfate ABC transporter substrate‑binding protein CysP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,536,106	0	T	C	100.0%	119.5 / NA	34	E261G (GAA→GGA)	cysP	thiosulfate/sulfate ABC transporter substrate‑binding protein CysP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/12);  total (22/12)

GTTATTCACGCGGTAGTAATAGTCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTTCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTGGTTTTCGGAATCACCACTTCAA  >  NZ_CP009273/2536021‑2536190                                                                                      |                                                                                     gTTATTCACGCGGTAGTAATAGTCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGt                                                                                  <  1:156002/90‑1 (MQ=255) gTTATTCACGCGGTAGTAATAGTCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGt                                                                                  <  1:871172/90‑1 (MQ=255)         cgcgGTAGTAATAGTCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTgg                                                                          >  2:592363/1‑90 (MQ=255)          gcgGTAGTAATAGTCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGc                                                                         >  2:519461/1‑90 (MQ=255)                     aGTCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGtttt                                                              >  2:251357/1‑90 (MQ=255)                       tCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTa                                                            >  2:523229/1‑90 (MQ=255)                       tCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTa                                                            >  1:318727/1‑90 (MQ=255)                       tCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTa                                                            >  2:336556/1‑90 (MQ=255)                       tCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTa                                                            >  2:201776/1‑90 (MQ=255)                       tCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTa                                                            >  1:758204/1‑90 (MQ=255)                       tCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTa                                                            >  1:808409/1‑90 (MQ=255)                       gcGGTGATGACGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCCTTGGCCTGCACGTTTTTa                                                            >  2:474158/2‑90 (MQ=255)                         ggTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATc                                                          <  2:836665/90‑1 (MQ=255)                         ggTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATc                                                          <  1:336556/90‑1 (MQ=255)                          gTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCa                                                         >  2:507828/1‑90 (MQ=255)                               gatgGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCa                                                    >  2:21043/1‑90 (MQ=255)                                    tttGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCa                                               >  2:736/1‑90 (MQ=255)                                      tGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCa                                                                    >  1:258515/1‑67 (MQ=255)                                      tGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCa                                                                    <  2:258515/67‑1 (MQ=255)                                            tGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAAt                                       >  2:52859/1‑90 (MQ=255)                                                  cTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGcc                                 <  2:758204/90‑1 (MQ=255)                                                            aGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTgg                       >  1:911198/1‑90 (MQ=255)                                                            aGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTgg                       >  1:216994/1‑90 (MQ=255)                                                            aGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTgg                       >  2:384171/1‑90 (MQ=255)                                                            aGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTgg                       >  2:116795/1‑90 (MQ=255)                                                             gTTCAGATAGGCTTTGGCGGC‑TTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTGGtt                     >  1:694455/1‑90 (MQ=255)                                                                 aGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTGGTTTTc                  >  1:111369/1‑90 (MQ=255)                                                                     aGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTGGTTTTCGGaa              <  1:736/90‑1 (MQ=255)                                                                       gCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTGGTTTTCGGAATc            >  2:822154/1‑90 (MQ=255)                                                                          ttGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTGGTTTTCGGAATcacc         <  1:289369/90‑1 (MQ=255)                                                                          ttGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTGGTTTTCGGAATcacc         <  2:24167/90‑1 (MQ=255)                                                                          ttGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTGGTTTTCGGAATcaac         <  1:504503/90‑3 (MQ=255)                                                                                 cTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTGGTTTTCGGAATCACCACTTCaa  <  1:523229/90‑1 (MQ=255)                                                                                 cTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTGGTTTTCGGAATCACCACTTCaa  <  2:733301/90‑1 (MQ=255)                                                                                      |                                                                                     GTTATTCACGCGGTAGTAATAGTCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTTCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTGGTTTTCGGAATCACCACTTCAA  >  NZ_CP009273/2536021‑2536190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑