Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,739,096	T→C	E61G (GAA→GGA)	rimM ←	ribosome maturation factor RimM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,739,096	0	T	C	100.0%	83.3 / NA	24	E61G (GAA→GGA)	rimM	ribosome maturation factor RimM
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/11);  total (13/11)

AATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTG  >  NZ_CP009273/2739013‑2739176                                                                                    |                                                                                 aaTTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCttccagcttcccagct                                                                            <  2:119810/90‑1 (MQ=255) aaTTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCttccagcttcccagct                                                                            <  1:545184/90‑1 (MQ=255)     cGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTCCCAGCTGGAc                                                                        >  2:663758/1‑90 (MQ=255)               ttCGCCGCATCCCGATCGCCACCCCCTTTCAGCTTGAGGATCATGTCCTGATTGTGGTGCTTCCATCTCCCCAGCGGGACTTGCTCCCAc                                                              <  2:112686/90‑1 (MQ=255)                    cgcATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGAcc                                                         <  1:562555/90‑1 (MQ=255)                     gcATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGAccc                                                        <  2:191403/90‑1 (MQ=255)                                gTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGAt                                             <  2:442154/90‑1 (MQ=255)                                gTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGAt                                             >  1:416934/1‑90 (MQ=255)                                gTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGAt                                             <  1:531353/90‑1 (MQ=255)                                gTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGAt                                             <  1:874093/90‑1 (MQ=255)                                  cAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGAt                                             >  2:693285/1‑88 (MQ=255)                                  cAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGAt                                             <  1:693285/88‑1 (MQ=255)                                       ccTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAg                                      >  1:129240/1‑90 (MQ=255)                                                ttGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAg                             <  2:816278/90‑1 (MQ=255)                                                        cATGTCCTGATTGTGGTGCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAAt                     >  1:410933/1‑90 (MQ=255)                                                        cATGTCCTGATTGTGGTGCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAAt                     >  2:681147/1‑90 (MQ=255)                                                               tGATTGTGGTGCTTCCAGCTTCCTAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCg              >  2:270335/1‑90 (MQ=255)                                                               tGATTGTGGTGCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCg              >  1:184938/1‑90 (MQ=255)                                                                gATTGTGGTGCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCg                                                       >  1:697877/1‑48 (MQ=255)                                                                gATTGTGGTGCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCg                                                       <  2:697877/48‑1 (MQ=255)                                                                gATTGTGGTGCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGAt                                             <  1:891802/58‑1 (MQ=255)                                                                gATTGTGGTGCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGAt                                             >  2:891802/1‑58 (MQ=255)                                                                gATTGTGGTGCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCgg             <  1:785810/90‑1 (MQ=255)                                                                gATTGTGGTGCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCgg             >  1:104952/1‑90 (MQ=255)                                                                    gtggtgCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTc         >  1:280488/1‑90 (MQ=255)                                                                      ggtgCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCtt       >  1:299748/1‑90 (MQ=255)                                                                           ttccagcttcccagctGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTg  >  2:903709/1‑90 (MQ=255)                                                                                    |                                                                                 AATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTG  >  NZ_CP009273/2739013‑2739176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑