Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,753,169	T→C	S276P (TCA→CCA)	yfjI →	YfjI family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,753,169	0	T	C	100.0%	77.4 / NA	22	S276P (TCA→CCA)	yfjI	YfjI family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/10);  total (12/10)

CAAAGGGGATTGGATTTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAA‑TTTCCAACCCAATTTTTTCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATGAGAGCATCATTAAAATTAATG  >  NZ_CP009273/2753083‑2753241                                                                                        |                                                                         cAAAGGGGATTGGATTTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAA‑TTTCCAACCCAATTTTTCCaa                                                                       >  2:40408/1‑90 (MQ=255)         attggattTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAA‑TTTCCAACCCAATTTTTCCAAATGAACAt                                                               >  2:723453/1‑90 (MQ=255)         attggattTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAA‑TTTCCAACCCAATTTTTCCAAATGAACAt                                                               >  1:549138/1‑90 (MQ=255)                       aCGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAA‑TTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCa                                                 >  1:307908/1‑90 (MQ=255)                          gTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAA‑TTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCa                                              >  2:869128/1‑90 (MQ=255)                           tGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAA‑TTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCaa                                             <  1:497188/90‑1 (MQ=255)                           tGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAA‑TTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCaa                                             <  2:549138/90‑1 (MQ=255)                            gCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAATTTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCaa                                             <  1:92089/90‑1 (MQ=255)                                    tgCCAGCCTGCTTCAACACAAGGTAACAGAAAAA‑TTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCaa                                             <  1:243558/81‑1 (MQ=255)                                    tgCCAGCCTGCTTCAACACAAGGTAACAGAAAAA‑TTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCaa                                             >  2:243558/1‑81 (MQ=255)                                    tgCCAGCCTGCTTCAACACAAGGTAACAGAAAAA‑TTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATg                                    >  1:920131/1‑90 (MQ=255)                                    tgCCAGCCTGCTTCAACACAAGGTAACAGAAAAA‑TTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATg                                    >  1:525402/1‑90 (MQ=255)                                      ccAGCCTGCTTCAACACAAGGTAACAGAAAAA‑TTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGa                                  >  1:761816/1‑90 (MQ=255)                                         gCCTGCTTCAACACAAGGTAACAGAAAAA‑TTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAAt                               >  2:187602/1‑90 (MQ=255)                                                      caAGGTAACAGAAAAA‑TTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATGAGAGc                  <  1:599605/90‑1 (MQ=255)                                                       aaGGTAACAGAAAAA‑TTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATGAGAGca                 <  1:905721/90‑1 (MQ=255)                                                           tAACAGAAAAA‑TTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATGAGAGcatcat             >  1:779667/1‑90 (MQ=255)                                                            aaCAGAAAAA‑TTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATGAGAGCATCAtt            <  1:678398/90‑1 (MQ=255)                                                             aCAGAAAAA‑TTTCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATGAGAGCATCATTa           >  1:72927/1‑90 (MQ=255)                                                                       tttCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATGAGAGCATCATTAAAATTAATg  <  2:235036/90‑1 (MQ=255)                                                                       tttCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATGAGAGCATCATTAAAATTAATg  <  1:723453/90‑1 (MQ=255)                                                                       tttCCAACCCAATTTTTCCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATGAGAGCATCATTAAAATTAATg  <  1:110956/90‑1 (MQ=255)                                                                                        |                                                                         CAAAGGGGATTGGATTTTTTGCACGGTGCCTCATGTGCCAGCCTGCTTCAACACAAGGTAACAGAAAAA‑TTTCCAACCCAATTTTTTCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATGAGAGCATCATTAAAATTAATG  >  NZ_CP009273/2753083‑2753241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑