| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,597,860 | C→T | T36T (ACC→ACT) | rsmD → | 16S rRNA (guanine(966)‑N(2))‑methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,597,860 | 0 | C | T | 82.3% | 37.2 / ‑0.1 | 17 | T36T (ACC→ACT) | rsmD | 16S rRNA (guanine(966)‑N(2))‑methyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (2/1); new base T (10/4); total (12/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCCAAATCCGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACCGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCATTGTTGACGCCCAATGTCTGGATTGCTTCGCCGG > NZ_CP009273/3597780‑3597937 | ggCCAAATCCGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACTGACCGCGTa < 1:348851/90‑1 (MQ=255) ccGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACTGACCGCGTACGCGAAAc < 2:901140/90‑1 (MQ=255) ggCGGGCAGTGGCGAGGCCGGAAACTCCCGGTTCCTGATAACCCCGGGCTGCGCCCCACCCCTGACCCCGTACGCGAAACGTTGTTTAAc > 2:808734/1‑90 (MQ=255) gggCAGGGGCGAGGCCCTAAACTCCCGGGTCCTTAAAGCCCACGGCTTCGCCCCACCACCGACCGCGGACGCGGAACGCTTTTTTAatgg > 2:331545/1‑90 (MQ=255) gCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACTGACCGCGTACGCGAAACGTTGTTTAActggct < 1:78183/90‑1 (MQ=255) gTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACTGACCGCGTACGCGAAACGTTGTTTAACtggctggc > 1:834068/1‑90 (MQ=255) ggCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACTGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTc > 2:607096/1‑90 (MQ=255) ggCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACCGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTc > 1:794972/1‑90 (MQ=255) tAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACTGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCAttgt > 1:610115/1‑90 (MQ=255) ccGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACTGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCATTGTTGACGcc > 1:177679/1‑90 (MQ=255) ggTTCCTGATAGCCCAGGTCTGCGCCCCACCACTGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCATTGTTGACGCCCa < 1:448103/90‑1 (MQ=255) tCCTGATAGCCCAGGTCTGCGCCCCACCACCGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCATTGTTGACGCCCAATg < 2:794972/90‑1 (MQ=255) gCCCAGGTCTGCGCCCCACCACTGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCCCCGGTCATTGTTGACGCCCAATGTCTGGAtt > 2:41981/1‑90 (MQ=255) cAGGTCTGCGCCCCACCACTGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCATTGTTGACGCCCAATGTCTGGATTGCt > 2:684980/1‑90 (MQ=255) aGGTCTGCGCCCCACCACTGACCGCGTACGCGAAACGTTGTTCAACTGGCTGGCTCCGGTCATTGTTGACGCCCAATGTCTGGATTGCtt > 2:790117/1‑90 (MQ=255) gcgcCCCACCACTGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCATTGTTGACGCCCAATGTCTGGATTGCTTCGCCgg > 2:115307/1‑90 (MQ=255) gcgcCCCACCACTGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCATTGTTGACGCCCAATGTCTGGATTGCTTCGCCgg > 1:249820/1‑90 (MQ=255) | GGCCAAATCCGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACCGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCATTGTTGACGCCCAATGTCTGGATTGCTTCGCCGG > NZ_CP009273/3597780‑3597937 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |