| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,679,062 | A→C | *1158E (TAA→GAA) | bcsC ← | cellulose synthase complex outer membrane protein BcsC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,679,062 | 0 | A | C | 95.0% | 133.2 / ‑0.8 | 40 | *1158E (TAA→GAA) | bcsC | cellulose synthase complex outer membrane protein BcsC |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base C (16/22); total (17/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.49e-01 | |||||||||||
ATTGTCTCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTACCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGC > NZ_CP009273/3678979‑3679144 | aTTGTCTCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTCCCAGTc < 2:893722/90‑1 (MQ=255)aTTGTCTCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTCCCAGTc < 1:152069/90‑1 (MQ=255)aTTGTCTCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTCCCAGTc < 1:880477/90‑1 (MQ=255)aTTGTCTCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTCCCAGTc < 2:573304/90‑1 (MQ=255)aTTGTCTCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTCCCAGTc < 2:721649/90‑1 (MQ=255)aTTGTCTCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTCCCAGTc < 2:817879/90‑1 (MQ=255) ctcCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTCCCAGTCGGCGt < 2:156670/90‑1 (MQ=255) ctcCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTCCCAGTCGGCGt > 1:864256/1‑90 (MQ=255) ccccccGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTACCAGTCGGCGTaa < 2:458510/90‑1 (MQ=255) ccGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTCCCAGTCGGCGTAAGGTa < 2:838552/90‑1 (MQ=255) aTCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTCCCAGTCGGCGTAAGGTATCAGCg > 1:576306/1‑90 (MQ=255) gATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTCCCAGTCGGCGTAAGGTATCAGCGGCTgcg > 2:79294/1‑90 (MQ=255) aTTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTCCCAGTCGGCGTAAGGTATCAGCGGCTgcgg < 2:676326/90‑1 (MQ=255) ccgccCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTCCCAGTCGGCGTAAGGTATCAGCGGCTGcggcggg > 2:543104/1‑89 (MQ=255) ccgccCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTCCCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGt > 2:804787/1‑90 (MQ=255) ccgccCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTCCCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGt > 1:413532/1‑90 (MQ=255) cgccCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTCCCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTa < 1:384378/90‑1 (MQ=255) cgccCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTCCCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTa < 2:553771/90‑1 (MQ=255) cccGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTCCCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTaaa < 2:726678/90‑1 (MQ=255) aCCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTCCCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGGGAATTCAAGTcc > 2:864400/1‑89 (MQ=255) cccGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTCCCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCAc > 1:162831/1‑90 (MQ=255) gATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTCCCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCAcccc > 2:477900/1‑89 (MQ=255) gATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTCCCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCt > 1:727623/1‑90 (MQ=255) gATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTACCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCt > 1:1293/1‑90 (MQ=255) aTCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTCCCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTg > 2:15623/1‑90 (MQ=255) aTCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTCCCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTg > 1:864404/1‑90 (MQ=255) cAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTCCCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCAt > 1:645102/1‑90 (MQ=255) cGGCATTAAGAGAGGCGCTATCTGAAAACTTCCCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCcg < 1:69812/90‑1 (MQ=255) cATTAAGAGAGGCGCTATCTGAAAACTTCCCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCcggcg > 1:803936/1‑90 (MQ=255) aTTAAGAGAGGCGCTATCTGAAAACTTCCCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCcggcgg < 2:864404/90‑1 (MQ=255) ttAAGAGAGGCGCTATCTGAAAACTTCCCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCcggcggc < 1:15623/90‑1 (MQ=255) gagagGCGCTATCTGAAAACTTCCCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGaa < 2:115107/90‑1 (MQ=255) agGCGCTATCTGAAAACTTCCCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTAAAt < 2:511604/58‑1 (MQ=255) agGCGCTATCTGAAAACTTCCCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTAAAt > 1:511604/1‑58 (MQ=255) agGCGCTATCTGAAAACTTCCCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCcggcggcgg > 2:117623/1‑85 (MQ=255) agGCGCTATCTGAAAACTTCCCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCcggcggcgg < 1:117623/85‑1 (MQ=255) ggCGCTATCTGAAAACTTCCCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAac < 1:672941/90‑1 (MQ=255) gcgcTATCTGAAAACTTCCCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAacg < 1:202474/90‑1 (MQ=255) gcgcTATCTGAAAACTTCCCAGTCGGCGTAAGGTATCAGCGGCTGAGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAacg < 2:763412/90‑1 (MQ=255) aaaaCTTCCCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGc < 1:874215/90‑1 (MQ=255) | ATTGTCTCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTACCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGC > NZ_CP009273/3678979‑3679144 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |