| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,922,863 | A→G | V34A (GTC→GCC) | viaA ← | ATPase RavA stimulator ViaA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,922,863 | 0 | A | G | 97.6% | 141.0 / ‑4.3 | 42 | V34A (GTC→GCC) | viaA | ATPase RavA stimulator ViaA |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (18/23); total (19/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
CTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAG‑‑GCGTGGGAATTTTTCAAAGAAGACCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGATCAATCCCTCTTCGCTGACGGCCAGCATCACATTAAGCGTATCC > NZ_CP009273/3922777‑3922952 | cTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAG‑‑GCGTGGGAATTTTTCAAAGAAGGCCg < 1:164050/90‑1 (MQ=255) ccGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAG‑‑GCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTg < 2:559564/90‑1 (MQ=255) gACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAG‑‑GCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCg > 2:70448/1‑90 (MQ=255) gACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAG‑‑GCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCg > 1:635371/1‑90 (MQ=255) gACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAG‑‑GCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCg > 1:609781/1‑90 (MQ=255) gACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAG‑‑GCGTGGGAATTTTTCAAAGAAGACCGCCAGCTGCg > 2:878738/1‑90 (MQ=255) tGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAG‑‑GCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTg < 1:251835/90‑1 (MQ=255) tGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAG‑‑GCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTg < 1:183232/90‑1 (MQ=255) tGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAG‑‑GCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTg > 2:3527/1‑90 (MQ=255) cgcAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTg < 2:35916/90‑1 (MQ=255) gcTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAG‑‑GCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCcagc < 2:591989/90‑1 (MQ=255) tCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAG‑‑GCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGt > 1:712228/1‑90 (MQ=255) aGCGGGGAACATCATCAGTGATTGCCGCCTTCAG‑‑GCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCgatga > 2:579651/1‑90 (MQ=255) aGCGGGGAACATCATCAGTGATTGCCGCCTTCAG‑‑GCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCgatga > 2:428582/1‑90 (MQ=255) gggggAACATCATCAGTGATTGCCGCCTTCAG‑‑GCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGatc < 2:364804/89‑1 (MQ=255) gggAACATCATCAGTGATTGCCGCCTTCAG‑‑GCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGatcat < 1:626499/90‑1 (MQ=255) atcatcAGTGATTGCCGCCTTCAG‑‑GCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTc > 2:280131/1‑90 (MQ=255) caccaGTGATTGCCGCCTTCAG‑‑GCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGa < 2:349049/87‑1 (MQ=255) tcaGTGATTGCCGCCTTCAG‑‑GCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGATc > 1:243366/1‑90 (MQ=255) aGTGATTGCCGCCTTCAG‑‑GCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGATCaa > 2:564514/1‑90 (MQ=255) aGTGATTGCCGCCTTCAG‑‑GCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGATCaa > 1:177275/1‑90 (MQ=255) gTGATTGCCGCCTTCAG‑‑GCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGATCAAt < 2:590571/90‑1 (MQ=255) tGATTGCCGCCTTCAG‑‑GCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGATCAATc < 1:181639/90‑1 (MQ=255) gATTGCCGCCTTCAG‑‑GCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGatcat < 2:729899/75‑1 (MQ=255) gATTGCCGCCTTCAG‑‑GCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGatcat > 1:729899/1‑75 (MQ=255) gATTGCCGCCTTCAG‑‑GCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGATCAATcc > 1:585976/1‑90 (MQ=255) aTTGCCGCCTTCAG‑‑GCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGATCAATccc > 2:46731/1‑90 (MQ=255) ttGCCGCCTTCAG‑‑GCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCgg > 1:882544/1‑49 (MQ=255) ttGCCGCCTTCAG‑‑GCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCgg < 2:882544/49‑1 (MQ=255) ccgccTTCAG‑‑GCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGATCAATCCCTCtt < 1:479686/90‑1 (MQ=255) tCAG‑‑GCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGATCAATCCCTCTTCGCTGa < 1:184988/90‑1 (MQ=255) cGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGATCAATCCCTCTTCGCTGACGGcc < 1:663913/90‑1 (MQ=255) cGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGATCAATCCCTCTTCGCTGACGGcc < 1:660766/90‑1 (MQ=255) gTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGATCAATCCCTCTTCGCTGACGGCCa > 1:615559/1‑90 (MQ=255) gggAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGATCAATCCCTCTTCGCTGACGGCCAGc > 1:194538/1‑90 (MQ=255) ggAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGATCAATCCCTCTTCGCTGACGGCCAGCa < 1:401001/90‑1 (MQ=255) aaTTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGATCAATCCCTCTTCGCTGACGGTCAGCATc < 2:142923/90‑1 (MQ=255) aaTTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGATCAATCCCTCTTCGCTGACGGCCAGCATc < 2:447160/90‑1 (MQ=255) aaTTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGATCAATCCCTCTTCGCTGACGGCCAGCATc < 1:636915/90‑1 (MQ=255) aaTTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGATCAATCCCTCTTCGCTGACGGCCAGCATc < 2:71445/90‑1 (MQ=255) tttttCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGATCAATCCCTCTTCGCTGACGGCCAGCATcac > 1:641378/1‑90 (MQ=255) ttCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGATCAATCCCTCTTCGCTGACGGCCAGCATCACAtt < 1:67189/90‑1 (MQ=255) aCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGATCAATCCCTCTTCGCTGACGGCCAGCATCACATTAAGCGTATcc < 1:878738/90‑1 (MQ=255) | CTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAG‑‑GCGTGGGAATTTTTCAAAGAAGACCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGATCAATCCCTCTTCGCTGACGGCCAGCATCACATTAAGCGTATCC > NZ_CP009273/3922777‑3922952 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |