Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,004,326	T→G	V256G (GTG→GGG)	yigL →	sugar/pyridoxal phosphate phosphatase YigL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,004,326	0	T	G	100.0%	137.6 / NA	39	V256G (GTG→GGG)	yigL	sugar/pyridoxal phosphate phosphatase YigL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (12/27);  total (12/27)

AGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATA  >  NZ_CP009273/4004240‑4004410                                                                                       |                                                                                     aGGCTGCATTATGGGCAGTGCGCATCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGcc                                                                                   <  2:110614/90‑1 (MQ=255)      gCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCAtt                                                                              >  1:852934/1‑90 (MQ=255)        aTTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTAt                                                                            <  2:695011/90‑1 (MQ=255)          tATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCt                                                                          <  2:769762/90‑1 (MQ=255)          tATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCt                                                                          <  1:576708/90‑1 (MQ=255)             gggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCg                                                                       <  1:502395/90‑1 (MQ=255)              ggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGt                                                                      <  1:665475/90‑1 (MQ=255)               gCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTa                                                                     <  1:646437/90‑1 (MQ=255)                cAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTaa                                                                    >  2:559895/1‑90 (MQ=255)                 aGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTaaa                                                                   >  1:848748/1‑90 (MQ=255)                  gTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAAc                                                                  >  2:264603/1‑90 (MQ=255)                  gTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAAc                                                                  <  1:65923/90‑1 (MQ=255)                  gTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAAc                                                                  <  2:821260/90‑1 (MQ=255)                   tGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAAct                                                                 <  1:563569/90‑1 (MQ=255)                   tGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAAct                                                                 >  2:57835/1‑90 (MQ=255)                    gcgcACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAActc                                                                >  2:214377/1‑90 (MQ=255)                       cACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAACTCTAt                                                             >  2:310897/1‑90 (MQ=255)                            gCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAACTCTATTTATc                                                        <  1:703711/90‑1 (MQ=255)                            gCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAACTCTATTTATc                                                        >  1:455045/1‑90 (MQ=255)                                 tGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAACTCTATTTATCGTAAt                                                   <  1:316565/90‑1 (MQ=255)                                  gAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAACTCTATTTATCGTAATc                                                  <  1:253844/90‑1 (MQ=255)                                  gAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAACTCTATTTATCGTAATc                                                  <  2:40142/90‑1 (MQ=255)                                  gAAAGACCTTCATCCCGAGCTGGAAGTAATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAACTCTATTTATCGTAATc                                                  <  2:326448/90‑1 (MQ=255)                                      gACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTc                                              <  1:383958/90‑1 (MQ=255)                                      gACCTTAATCCCGAGCTGGTTGGGATTGGCACTAATGCCGACGACGCGGGGCCGCTTAATCTTCGTAGAGTCTCTTTATCGTAATGGTTc                                              >  2:876883/1‑90 (MQ=255)                                          ttCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAACTCTATTTATCGTAATc                                                  >  1:309899/1‑82 (MQ=255)                                          ttCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAACTCTATTTATCGTAATc                                                  <  2:309899/82‑1 (MQ=255)                                                  gAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCa                                  <  1:739440/90‑1 (MQ=255)                                                        gAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTc                            <  2:455045/90‑1 (MQ=255)                                                                    aCTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACCTGATACtt                <  2:920567/90‑1 (MQ=255)                                                                     cTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTc               <  2:289949/90‑1 (MQ=255)                                                                       aaTGCCGACGACGCGGGGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGc             >  2:790224/1‑90 (MQ=255)                                                                         tGCCGACGACGCGGGGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTa           <  1:457551/90‑1 (MQ=255)                                                                         tGCCGACGACGCGGGGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTa           <  1:358831/90‑1 (MQ=255)                                                                          gCCGACGACGCGGGGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTAc          <  2:154551/90‑1 (MQ=255)                                                                            cgacgaCGCGGGGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACaa        >  2:92741/1‑90 (MQ=255)                                                                                 acgCGGGGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGAt   <  2:401416/90‑1 (MQ=255)                                                                                 acgCGGGGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGAt   <  1:564556/90‑1 (MQ=255)                                                                                  cgcgGGGCCGCATTATCTGCGTAAACTCTATTTATCGTAATAGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATa  <  1:518157/90‑1 (MQ=255)                                                                                       |                                                                                     AGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATA  >  NZ_CP009273/4004240‑4004410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑