| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,336,254 | T→G | D297A (GAC→GCC) | fumB ← | class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,336,254 | 0 | T | G | 95.5% | 72.3 / ‑3.3 | 22 | D297A (GAC→GCC) | fumB | class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (13/8); total (13/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.78e-01 | |||||||||||
CGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATC > NZ_CP009273/4336166‑4336339 | cGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTc < 2:239203/90‑1 (MQ=255) gCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGGCg > 1:163593/1‑90 (MQ=255) aTGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGGCGTGcgcg < 1:466637/90‑1 (MQ=255) tGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGGCGTGCGCGa < 2:161692/90‑1 (MQ=255) aCGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGGCGTGCGCGAAGTa > 2:647220/1‑90 (MQ=255) aCGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGGCGTGCGCGAAGTa > 1:452551/1‑90 (MQ=255) aCGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGGCGTGCGCGAAGTa > 2:462313/1‑90 (MQ=255) gTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGGCGTGCGCGAAGTAttt < 1:305233/90‑1 (MQ=255) gTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGGCGTGCGCGAAGTAttt < 1:647220/90‑1 (MQ=255) cAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGGTAACGCGAATGGCGTGCGCGAAGTATTTAc > 2:30354/1‑90 (MQ=255) gCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGGCGTGCGCGAAGTATTTACCg > 1:80234/1‑90 (MQ=255) gCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGGCGTGCGCGAAGTATTTACCGCCaaa > 1:18272/1‑90 (MQ=255) gACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGGCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAg > 1:429923/1‑90 (MQ=255) ggATGCGCCGTGACGTGGCAGACGGATAACGCGAATGGCGTGCGCGAAGTCTTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGc < 1:760784/90‑1 (MQ=255) aTGCGCCGTGACGTGGCAGACGGATAACGCGAATGGCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCct > 1:347617/1‑90 (MQ=255) cgcCGTGACGTGGCAGACGGATAACGCGAATGGCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCtt < 1:122242/90‑1 (MQ=255) tGACGTGGCAGACGGATAACGCGAATGGCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCcagc > 2:188340/1‑90 (MQ=255) gACGGATAACGCGAATGGCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGtt < 1:9013/90‑1 (MQ=255) gATAACGCGAATGGCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCAg > 1:73554/1‑90 (MQ=255) gAATGGCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCca > 1:569691/1‑66 (MQ=255) gAATGGCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCca < 2:569691/66‑1 (MQ=255) aaTGGCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATc > 2:693185/1‑90 (MQ=255) | CGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATC > NZ_CP009273/4336166‑4336339 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |