| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,421,635 | C→G | A56P (GCA→CCA) | ytfE ← | iron‑sulfur cluster repair protein YtfE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,421,635 | 0 | C | G | 93.0% | 133.6 / 0.3 | 43 | A56P (GCA→CCA) | ytfE | iron‑sulfur cluster repair protein YtfE |
| Reads supporting (aligned to +/- strand): ref base C (1/2); new base G (12/28); total (13/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.22e-01 | |||||||||||
TGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCC > NZ_CP009273/4421550‑4421720 | tGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGt > 1:452171/1‑90 (MQ=255) ggTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGtt < 2:873726/90‑1 (MQ=255) aGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAg < 1:481223/90‑1 (MQ=255) gcgcACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAGc > 2:57947/1‑90 (MQ=255) atgatATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCAATg > 2:356871/1‑90 (MQ=255) tgatATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTATTCAGCGAGCTTTGGCAGTTCAGCTTCAATGa < 1:462177/90‑1 (MQ=255) gatATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCAATGAc < 1:619522/90‑1 (MQ=255) ggTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCAATGACCTCaa < 1:356871/90‑1 (MQ=255) ggTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCAATGACCTCaa < 1:38074/90‑1 (MQ=255) ggTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCaa < 1:439910/90‑1 (MQ=255) gTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCAATGACCTCAAc > 1:195419/1‑90 (MQ=255) cGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCAATg > 2:427009/1‑80 (MQ=255) cGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCAATg < 1:427009/80‑1 (MQ=255) atgatTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCAATGACCTCAACATcc < 2:147718/90‑1 (MQ=255) atgatTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCAATGACCTCAACATcc < 2:240008/90‑1 (MQ=255) tgatTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCAATGACCTCAACATCCa < 2:452171/90‑1 (MQ=255) tttCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCAATGACCTCAACATCCAGtt < 1:403857/90‑1 (MQ=255) ttCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCAATGACCTCAACATCCAGTTc > 2:50623/1‑90 (MQ=255) ttCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCAATGACCTCAACATCCAGTTc > 1:884685/1‑90 (MQ=255) ggCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTgccgc < 2:464216/90‑1 (MQ=255) cgcTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGccgccg < 1:728259/90‑1 (MQ=255) cTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCcgcg > 2:354279/1‑90 (MQ=255) ccAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCgcgcgcgc < 1:50623/90‑1 (MQ=255) ccAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCAATGACCTAAACATCCAGTTCTTTACGTGCCGCCgcgcgcgc < 1:629507/90‑1 (MQ=255) aGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCa > 2:294302/1‑90 (MQ=255) aTCGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTa > 2:173497/1‑90 (MQ=255) cGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCAATGCCCTCAACACCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTAcc < 2:160734/90‑1 (MQ=255) ttgttCAGCGAGCTTTGGCAGTTCAGCTTCAATGCCCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGcc < 2:42241/90‑1 (MQ=255) ttgttCAGCGAGCTTTGGCAGTCCAGCTTCAATGCCCCCACCACCCAGTTCTTTACGTGCCCCCGCGCGCGCCAGCGTCTGCTTACCGcc < 2:474378/90‑1 (MQ=255) ttgttCAGCGAGCTTCGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGcc > 2:188201/1‑90 (MQ=255) cAGCGAGCTTTGGCAGTTCAGCTTCAATGACCTCAACATCCATTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCAcagc < 2:195419/90‑1 (MQ=255) gCGAGCTTTGGCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCAcagcag < 1:16090/90‑1 (MQ=255) gCGAGCTTTGGCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCAcagcag > 2:425373/1‑90 (MQ=255) gCGAGCTTTGGCAGTTCAGCTTCAATGACCTCAACATCCAGTTC‑TTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGt > 2:772132/1‑90 (MQ=255) gCGAGCTTTGGAAGTTCAGCTTCAATGCCCTCAACTTCCAGTTCTTTACGTGCCGCAGCGCGCGCCAGCGTCTGCTTACCGCCAcagcag < 1:330143/90‑1 (MQ=255) cGAGCTTTGGCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGt < 1:808839/90‑1 (MQ=255) cGAGCTTTGGCAGTTCAGCTTCAATGACCTCAACACCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGt < 2:705549/90‑1 (MQ=255) gAGCTTTGGCAGTTCAGCTTAAATGCCCTCAACACCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTa < 2:425173/90‑1 (MQ=255) aGCTTTGGCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTaa < 1:564612/90‑1 (MQ=255) cTTTGGCAGTTCGGCTTCAATGACCTCAACATCCAGTTC‑TTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATcc < 1:772132/90‑1 (MQ=255) cTTTGGCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATc < 1:201820/90‑1 (MQ=255) cTTTGGCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATc < 1:294302/90‑1 (MQ=255) cTTTGCCAGTTCAGCTCCACTGCCCTCACCACCCAGTTTTTTCCGTCCCGCCGCCCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATc < 2:626772/90‑1 (MQ=255) | TGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCC > NZ_CP009273/4421550‑4421720 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |