| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,500,063 | T→G | intergenic (+113/+444) | BW25113_RS25830 → / ← fecE | hypothetical protein/Fe(3+) dicitrate ABC transporter ATP‑binding protein FecE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,500,063 | 0 | T | G | 97.9% | 170.2 / ‑3.7 | 48 | intergenic (+113/+444) | BW25113_RS25830/fecE | hypothetical protein/Fe(3+) dicitrate ABC transporter ATP‑binding protein FecE |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (22/25); total (22/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
TACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTC > NZ_CP009273/4499982‑4500148 | tacctaAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTa < 1:459398/90‑1 (MQ=255) ctaAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGt < 2:232341/90‑1 (MQ=255) aaGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTg > 1:907505/1‑90 (MQ=255) gtagCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTc > 2:249940/1‑90 (MQ=255) gtagCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTc > 2:396683/1‑90 (MQ=255) gtagCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTc < 2:87064/90‑1 (MQ=255) gtagCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTc > 1:685921/1‑90 (MQ=255) tagCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTCt > 2:43037/1‑90 (MQ=255) tagCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTCt > 1:348441/1‑90 (MQ=255) agCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTCTg < 2:262972/90‑1 (MQ=255) agCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTCTg > 2:300489/1‑90 (MQ=255) agCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTCTg < 1:629573/90‑1 (MQ=255) aaaCGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTCTGATc > 1:196170/1‑90 (MQ=255) aaCGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTCTGATCa < 1:434342/90‑1 (MQ=255) aCGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTCTGATCAt > 1:110311/1‑90 (MQ=255) aCGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTCTGATCAt > 1:379675/1‑90 (MQ=255) aCGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTCTGATCAt < 2:824597/90‑1 (MQ=255) cGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTg > 2:187961/1‑77 (MQ=255) cGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTg < 1:187961/77‑1 (MQ=255) gCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTCTGATCATTg > 2:323723/1‑90 (MQ=255) cacaCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTCTGATCATTGa > 2:650868/1‑90 (MQ=255) cacaCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTCTGATCATTGa > 1:908322/1‑90 (MQ=255) cacTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTCTGATCATTGAtt < 1:480271/90‑1 (MQ=255) ttttAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTCTGATCATTGATTTATc < 2:637925/90‑1 (MQ=255) ttttAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTCTGATCATTGATTTATc > 2:620197/1‑90 (MQ=255) ttttAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTCTGATCATTGATTTATc < 2:203543/90‑1 (MQ=255) gAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCaa < 2:704161/90‑1 (MQ=255) cGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCa < 1:650868/90‑1 (MQ=255) cGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCa < 1:546048/90‑1 (MQ=255) tGGTCAGAACCTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAAcc < 2:260228/90‑1 (MQ=255) ggTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCt < 2:379675/90‑1 (MQ=255) aGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCAtt < 2:908322/90‑1 (MQ=255) ttCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAAcc < 1:185332/68‑1 (MQ=255) ttCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAAcc > 2:185332/1‑68 (MQ=255) ttCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTcc > 2:55862/1‑90 (MQ=255) gTTCCATGGTTGCTGTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGa > 2:19083/1‑90 (MQ=255) gTTCCATGGTTGCTGTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGa < 1:855281/90‑1 (MQ=255) gTTCCATGGTTGCTGTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGa > 1:301392/1‑90 (MQ=255) gTTCCATGGTTGCTGTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGa > 2:724168/1‑90 (MQ=255) gTTCCATGGTTGCTGTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGa < 1:269831/90‑1 (MQ=255) ttCCATGGTTGCTGTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGaa > 2:749609/1‑90 (MQ=255) tCCATGGTTGCTGTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAAt > 2:672406/1‑90 (MQ=255) tGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTcc < 1:201959/90‑1 (MQ=255) tGGTTGCTGTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTcc < 2:468699/90‑1 (MQ=255) tGGTTGCTGTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTcc < 1:55862/90‑1 (MQ=255) tGGTTGCTGTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTcc < 1:300489/90‑1 (MQ=255) gTTGCTGTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGc < 2:337204/90‑1 (MQ=255) tGCTGTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGctc < 1:749609/90‑1 (MQ=255) | TACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTC > NZ_CP009273/4499982‑4500148 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |