Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	575,614	A→G	intergenic (+72/+93)	BW25113_RS02885 → / ← ylcI	hypothetical protein/YlcI/YnfO family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	575,614	0	A	G	100.0%	133.6 / NA	37	intergenic (+72/+93)	BW25113_RS02885/ylcI	hypothetical protein/YlcI/YnfO family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/15);  total (22/15)

CTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAAC  >  NZ_CP009273/575528‑575697                                                                                       |                                                                                    cTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAg                                                                                  >  2:265892/1‑90 (MQ=255)             tGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGc                                                                      >  2:270095/1‑90 (MQ=255)              gATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCa                                                                     <  2:829939/90‑1 (MQ=255)              gATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCa                                                                     <  2:357866/90‑1 (MQ=255)                 cACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACt                                                                  <  2:514684/90‑1 (MQ=255)                  aCCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTc                                                                 >  1:57382/1‑90 (MQ=255)                        aCTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTAc                                                           >  1:853160/1‑90 (MQ=255)                            cTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCa                                                       >  1:833385/1‑90 (MQ=255)                             tAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCaa                                                      <  1:464233/90‑1 (MQ=255)                             tAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCaa                                                      >  1:196894/1‑90 (MQ=255)                             tAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCaa                                                      >  1:672197/1‑90 (MQ=255)                                tCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGc                                                   <  2:824621/90‑1 (MQ=255)                                   ttCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTAc                                                           <  2:553727/79‑1 (MQ=255)                                   ttCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTAc                                                           >  1:553727/1‑79 (MQ=255)                                   ttCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCt                                                >  1:738708/1‑90 (MQ=255)                                   ttCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCt                                                >  1:159944/1‑90 (MQ=255)                                   ttCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCt                                                <  1:39994/90‑1 (MQ=255)                                           actTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTa                                        >  2:294227/1‑90 (MQ=255)                                            ctTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTaa                                       >  2:393726/1‑90 (MQ=255)                                               aaCCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTg                                    <  1:44150/90‑1 (MQ=255)                                               aaCCTGTGACAGAGCCAACACGCAGTCAGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTg                                    <  2:414841/90‑1 (MQ=255)                                                   tgtgACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAAtt                                >  1:709514/1‑90 (MQ=255)                                                     tgACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGt                                     >  2:822680/1‑83 (MQ=255)                                                     tgACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGt                                     <  1:822680/83‑1 (MQ=255)                                                     tgACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTa                              >  1:850263/1‑90 (MQ=255)                                                     tgACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTa                              >  1:76476/1‑90 (MQ=255)                                                     tgACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTa                              >  2:526338/1‑90 (MQ=255)                                                      gACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTAc                             <  1:393726/90‑1 (MQ=255)                                                      gACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTAc                             <  1:199586/90‑1 (MQ=255)                                                          gagCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGt                                     <  2:182167/78‑1 (MQ=255)                                                          gagCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGt                                     >  1:182167/1‑78 (MQ=255)                                                          gagCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAata                         >  1:673027/1‑90 (MQ=255)                                                              cAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGt                     >  1:417679/1‑90 (MQ=255)                                                                acacGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTcc                   <  2:57382/90‑1 (MQ=255)                                                                        tCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGa           >  1:861369/1‑90 (MQ=255)                                                                         ctgtcactgtcaGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAg          <  1:714058/90‑1 (MQ=255)                                                                                 gtcaGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAAc  >  1:220012/1‑90 (MQ=255)                                                                                       |                                                                                    CTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAAC  >  NZ_CP009273/575528‑575697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑