| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 587,468 | A→C | D389E (GAT→GAG) | nrfB ← | cyclic di‑3',5'‑guanylate‑activated glycosyltransferase NrfB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 587,468 | 0 | A | C | 93.1% | 91.8 / ‑0.7 | 29 | D389E (GAT→GAG) | nrfB | cyclic di‑3',5'‑guanylate‑activated glycosyltransferase NrfB |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base C (12/15); total (13/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.28e-01 | |||||||||||
ATGCGGTTAACCATCAAACCAAAGTTTAGCCACAGCAGGGTCATTAACCATGCGCTGCCGCTGAAAATAGAAAGGAAATGCCAGGCATCGGGCCACAAACTTTCATACGCCAGCAACAGCAAAAGCTGGATCATCACCAGCATCGCGAGGAAGCTGACAAAGTTACTGATTGC > NZ_CP009273/587382‑587554 | aTGCGGTTAACCATCAAACCAAAGTTTAGCCACAGCAGGGTCATTAACCATGCGCTGCCGCTGAAAATAGAAAGGAAATGCCAGGCCTCg > 2:851309/1‑90 (MQ=255) acaGCAGGGTCATTAACCATGCGCTGCCGCTGAAAATAGAAAGGAAATGCCAGGCCTCGGGCCACAAACTTTCATACGCcagcaacagca < 2:410637/90‑1 (MQ=255) acaGCAGGGTCATTAACCATGCGCTGCCGCTGAAAATAGAAAGGAAATGCCAGGCCTCGGGCCACAAACTTTCATACGCcagcaacagca < 1:616605/90‑1 (MQ=255) cagcagGGTCATTAACCATGCGCTGCCGCTGAAAATAGAAAGGAAATGCCAGGCCTCGGGCCACAAACTTTCATACGCcagcaacagcaa > 2:498507/1‑90 (MQ=255) cagcagGGTCATTAACCATGCGCTGCCGCTGAAAATAGAAAGGAAATGCCAGGCCTCGGGCCACAAACTTTCATACGCcagcaacagcaa > 1:574891/1‑90 (MQ=255) aTTAACCATGCGCTGCCGCTGAAAATAGAAAGGAAATGCCAGGCCTCGGGCCACAAACTTTCATACGCCAGCAACAGCAAAAGCTGGatc > 1:191623/1‑90 (MQ=255) ttAACCATGCGCTGCCGCTGAAAATAGAAAGGAAATGCCAGGCCTCGGGCCACAAACTTTCATACGCCAGCAACAGCAAAAGCTGGatca < 1:667871/90‑1 (MQ=255) tAACCATGCGCTGCCGCTGAAAATAGAAAGGAAATGCCAGGCCTCGGGCCACAAACTTTCATACGCCAGCAACAGCAAAAGCTGGatcat > 2:332016/1‑90 (MQ=255) aTGCGCTGCCGCTGAAAATAGAAAGGAAATGCCAGGCCTCGGGCCACAAACTTTCATACGCCAGCAACAGCAAAAGCTGGat > 2:335658/1‑82 (MQ=255) aTGCGCTGCCGCTGAAAATAGAAAGGAAATGCCAGGCCTCGGGCCACAAACTTTCATACGCCAGCAACAGCAAAAGCTGGat < 1:335658/82‑1 (MQ=255) aTGCGCTGCCGCTGAAAATAGAAAGGAAATGCCAGGCCTCGGGCCACAAACTTTCATACGCCAGCAACAGCAAAAGCTGGATCATCACCa > 2:711143/1‑90 (MQ=255) aTGCGCTGCCGCTGAAAATAGAAAGGAAATGCCAGGCCTCGGGCCACAAACTTTCATACGCAAGCAACAGCAAAAGCTGGATCATCACCa > 2:67076/1‑90 (MQ=255) aTGCGCTGCCGCTGAAAATAGAAAGGAAATGCCAGGCATCGGGCCACAAACTTTCATACGCcagcaa > 2:288249/1‑67 (MQ=255) aTGCGCTGCCGCTGAAAATAGAAAGGAAATGCCAGGCATCGGGCCACAAACTTTCATACGCcagcaa < 1:288249/67‑1 (MQ=255) gcgcTGCCGCTGAAAATAGAAAGGAAATGCCAGGCCTCGGGCCACAAACTTTCATACGCCAGCAACAGCAAAAGCTGGATCATCACCAGc > 2:69562/1‑90 (MQ=255) cGCTGAAAATAGAAAGGAAATGCCAGGCCTCGGGCCACAAACTTTCATACGCCAGCAACAGCAAAAGCTGGATCATCACCAGCATCGCGa < 2:540299/90‑1 (MQ=255) aaaTAGAAAGGAAATGCCAGGCCTCGGGCCACAAACTTTCATACGCCAGCAACAGCAAAAGCTGGATCATCACCAGCATCGCGAGGAAGc < 1:140777/90‑1 (MQ=255) aGAAAGGAAATGCCAGGCCTCGGGCCACAAACTTTCATACGCCAGCAACAGCAAAAGCTGGATCATCACCAGCATCGCGAGGAAGCTGAc < 1:173094/90‑1 (MQ=255) aGAAAGGAAATGCCAGGCCTCGGGCCACAAACTTTCATACGCCAGCAACAGCAAAAGCTGGATCATCACCAGCATCGCGAGGAAGCTGAc < 2:280653/90‑1 (MQ=255) aaaGGAAATGCCAGGCCTCGGGCCACAAACTTTCATACGCCAGCAACAGCAAAAGCTGGATCATCACCAGCATCGCGAGGAAGCTGACaa < 1:674573/90‑1 (MQ=255) aaaGGAAATGCCAGGCCTCGGGCCACAAACTTTCATACGCCAGCAACAGCAAAAGCTGGATCATCACCAGCATCGCGAGGAAGCTGACaa < 1:332016/90‑1 (MQ=255) aaaTGCCAGGCCTCGGGCCACAAACTTTCATACGCCAGCAACAGCAAAAGCTGGATCATCACCAGCATCGCGAGGAAGCTGACAAAGTTa < 1:92750/90‑1 (MQ=255) aaTGCCAGGCCTCGGGCCACAAACTTTCATACGCCAGCAACAGCAAAAGCTGGATCATCACCAGCATCGCGAGGAAGCTGACAAAGTTAc < 1:117282/90‑1 (MQ=255) aaTGCCAGGCCTCGGGCCACAAACTTTCATACGCCAGCAACAGCAAAAGCTGGATCATCACCAGCATCGCGAGGAAGCTGACAAAGTTAc > 1:315707/1‑90 (MQ=255) aaTGCCAGGCCTCGGGCCACAAACTTTCATACGCCAGCAACAGCAAAAGCTGGATCATCACCAGCATCGCGAGGAAGCTGACAAAGTTAc < 1:116095/90‑1 (MQ=255) cAGGCCTCGGGCCACAAACTTTCATACGCCAGCAACAGCAAAAGCTGGATCATCACCAGCATCGCGAGGAAGCTGACAAAGTTACTGAtt > 2:40445/1‑90 (MQ=255) ggCCTCGGGCCACAAACTTTCATACGCCAGCAACAGCAAAAGCTg > 2:396769/1‑45 (MQ=255) ggCCTCGGGCCACAAACTTTCATACGCCAGCAACAGCAAAAGCTg < 1:396769/45‑1 (MQ=255) ggCCTCGGGCCACAAACTTTCATACGCCAGCAACAGCAAAAGCTGGATCATCACCAGCATCGCGAGGAAGCTGACAAAGTTACTGATTGc < 1:851309/90‑1 (MQ=255) | ATGCGGTTAACCATCAAACCAAAGTTTAGCCACAGCAGGGTCATTAACCATGCGCTGCCGCTGAAAATAGAAAGGAAATGCCAGGCATCGGGCCACAAACTTTCATACGCCAGCAACAGCAAAAGCTGGATCATCACCAGCATCGCGAGGAAGCTGACAAAGTTACTGATTGC > NZ_CP009273/587382‑587554 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |