Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	661,892	A→C	G594G (GGT→GGG)	mrdA ←	peptidoglycan DD‑transpeptidase MrdA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	661,892	0	A	C	100.0%	92.6 / NA	27	G594G (GGT→GGG)	mrdA	peptidoglycan DD‑transpeptidase MrdA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (12/15);  total (12/15)

TTCCGCAGGCAGATCGGTGTTGTTATCACCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCACCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTCACGTAAACGC  >  NZ_CP009273/661805‑661976                                                                                        |                                                                                     ttCCGCAGGCAGATCGGTGTTGTTATCACCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCaccccc                                                                                    >  1:614819/1‑90 (MQ=255)   ccGCAGGCAGATCGGTGTTGTTATCACCCAGCATAATGTGGTCGAGGATCTGGGGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGt                                                                                  <  1:267167/90‑1 (MQ=255)    cGCAGGCAGATCGGTGTTGTTATCCCCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGtt                                                                                 <  2:688154/90‑1 (MQ=255)                           cACCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCa                                                          >  2:252932/1‑90 (MQ=255)                           cACCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCa                                                          >  2:566422/1‑90 (MQ=255)                                aGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGc                                                     >  1:387464/1‑90 (MQ=255)                                aGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGc                                                     >  2:279613/1‑90 (MQ=255)                                aGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGc                                                     >  1:116849/1‑90 (MQ=255)                                  cATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCgg                                                   <  1:823973/90‑1 (MQ=255)                                  cATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTCCTCCAGAATCATGGCGACAGCCACTTGCgg                                                   <  2:45398/90‑1 (MQ=255)                                    tAATGTGGTCGAGGATCTGGCGCATCAGTGTCCCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGAt                                                 <  2:465839/90‑1 (MQ=255)                                    tAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGAt                                                 <  2:782032/90‑1 (MQ=255)                                    tAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGAt                                                 <  2:116849/90‑1 (MQ=255)                                    tAATGTGGTCGAGGATCTCGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGAt                                                 <  1:705901/90‑1 (MQ=255)                                           gTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTa                                          >  1:263901/1‑90 (MQ=255)                                               aGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGgc                                      >  2:881428/1‑90 (MQ=255)                                                     tGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAg                                >  2:524428/1‑90 (MQ=255)                                                          cATCAGTGTCCCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGt                           <  2:844057/90‑1 (MQ=255)                                                              aGTGTACCACCCGCCGGACCCGCCCCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGtcatc                       <  2:48571/90‑1 (MQ=255)                                                              aGTGTACCACCCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGtcatc                       <  2:726374/90‑1 (MQ=255)                                                              aGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGtcatc                       <  1:708659/90‑1 (MQ=255)                                                                      aaCCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTtgtg               >  1:226288/1‑90 (MQ=255)                                                                      aaCCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTtgtg               >  1:537642/1‑90 (MQ=255)                                                                         cgccgGCCCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTc            <  1:50741/90‑1 (MQ=255)                                                                         cgccgGACCCGCACCCCCGTTCTCCAGAATCCGGGCGCCAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTc            <  1:453955/90‑1 (MQ=255)                                                                         cgccgGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTc            >  1:8364/1‑90 (MQ=255)                                                                                   gCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTCACGTAAACGc  <  1:708737/90‑1 (MQ=255)                                                                                   gCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTCACGTAAACGc  >  1:672083/1‑90 (MQ=255)                                                                                        |                                                                                     TTCCGCAGGCAGATCGGTGTTGTTATCACCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCACCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTCACGTAAACGC  >  NZ_CP009273/661805‑661976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑