| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 901,740 | G→A | A157V (GCG→GTG) | ybjS ← | NAD(P)‑dependent oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 901,740 | 0 | G | A | 96.4% | 94.6 / ‑3.9 | 28 | A157V (GCG→GTG) | ybjS | NAD(P)‑dependent oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (17/10); total (18/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01 | |||||||||||
ATATGCGCCAGACGGGGAATAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGCGCAGAATAGTAAAGCGCGTTTGTGGATTCGCCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGCAAACTCGTTGGCGAAGCGGTGAGGGCGAAAATCT > NZ_CP009273/901653‑901823 | atatGCGCCAGACGGGGAATAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGCGCAGAATAGTAAAGCGCGTTTGTGGATTCAcc > 1:761958/1‑90 (MQ=255) tGCGCCAGACGGGGAATAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGCGCAGAATAGTAAAGCGCGTTTGTGGATTCACCTGc > 2:774453/1‑90 (MQ=255) gcgcCAGACGGGGAATAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGCGCAGAATAGTAAAGCGCGTTTGTGGATTCACCTGCg < 2:134650/90‑1 (MQ=255) cgcCAGACGGGGAATAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGCGCAGAATAGTAAAGCGCGTTTGTGGATTCACCTGCGa > 1:111342/1‑90 (MQ=255) gcCAGACGGGGAATAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGCGCAGAATAGTAAAGCGCGTTTGTGGATTCACCTGCGaa > 2:287145/1‑90 (MQ=255) gcCAGACGGGGAATAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGCGCAGAATAGTAAAGCGCGTTTGTGGATTCACCTGCGaa < 2:111342/90‑1 (MQ=255) gACGGGGAATAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGCGCAGAATAGTAAAGCGCGTTTGTGGATTCGCCTGCGAAAGCa > 2:662093/1‑90 (MQ=255) aTAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGCGCAGAATAGTAAAGCGCGTTTGTGGATTCACCTGCGAAAGCATATTGATc < 2:811501/90‑1 (MQ=255) tAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGCGCAGAATAGTAAAGCGCGTTTGTGGATTCACCTGCGAAAGCATATTGATCa > 1:184711/1‑90 (MQ=255) tttATCGTGCGGTCCGAACAGACTTTGTGGGCGCAGAATAGTAAAGCGCGTTTGTGGATTCACCTGCGAAAGCATATTGATCACttcttc > 2:229229/1‑90 (MQ=255) tATCGTGCGGTCCGAACAGACTTTGTGGGCGCAGAATAGTAAAGCGCGTTTGTGGATTCACCTGCGAAAGCATATTGATCACTTCTTCgc > 1:751450/1‑90 (MQ=255) tATCGTGCGGTCCGAACAGACTTTGTGGGCGCAGAATAGTAAAGCGCGTTTGTGGATTCACCTGCGAAAGCATATTGATCACTTCTTCgc > 2:21052/1‑90 (MQ=255) gTGCGGTCCGAACAGACTTTGTGGGCGCAGAATAGTAAAGCGCGTTTGTGGATTCACCTGCGAAAGCATATTGATCACTTCTTCgctggc < 2:135124/90‑1 (MQ=255) gTCCGAACAGACTTTGTGGGCGCAGAATAGTAAAGCGCGTTTGTGGATTCACCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCtt > 2:810274/1‑90 (MQ=255) gACTTTGTGGGCGCAGAATAGTAAAGCGCGTTTGTGGATTCACCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCggg > 2:335427/1‑90 (MQ=255) aCTTTGTGGGCGCAGAATAGTAAAGCGCGTTTGTGGATTCACCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGc > 1:328275/1‑90 (MQ=255) tttGTGGGCGCAGAATAGTAAAGCGCGTTTGTGGATTCACCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGCaa > 1:110753/1‑90 (MQ=255) tAGTAAAGCGCGTTTGTGGATTCACCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGCAAACTCGTTGGCGAAGc < 2:110753/90‑1 (MQ=255) aaGCGCGTTTGTGGATTCACCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGCAAACTCGTTGGCGAAGCGGTGa < 2:551722/90‑1 (MQ=255) aaGCGCGTTTGTGGATTCACCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGCAAACTCGTTGGCGAAGCGGTGa < 2:646314/90‑1 (MQ=255) aGCGCGTTTGTGGATTCACCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGCAAACTCGTTGGCGAAGCGGTGAg < 2:296286/90‑1 (MQ=255) gcgcGTTTGTGGATTCACCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGCAAACTCGTTGGCGAAGCGGTGAgg > 2:375046/1‑90 (MQ=255) gcgcGTTTGTGGATTCACCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGCAAACTCGTTGGCGAAGCGGTGAgg > 2:533460/1‑90 (MQ=255) gcgcGTTTGTGGATTCACCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGCAAACTCGTTGGCGAAGCGGTGAgg > 1:192607/1‑90 (MQ=255) gcgcGTTTGTGGATTCACCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGCAAACTCGTTGGCGAAGCGGTGAgg > 2:682879/1‑90 (MQ=255) gcgTTTGTGGATTCACCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGCAAACTCGTTGGCGAAGCGGTGAGGGc < 1:794226/90‑1 (MQ=255) gTTTGTGGATTCACCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGCAAACTCGTTGGCGAAGCGGTGAGGGCGa > 2:206571/1‑90 (MQ=255) ggATTCACCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGCAAACTCGTTGGCGAAGCGGTGAGGGCGAAAAtct < 1:639316/90‑1 (MQ=255) | ATATGCGCCAGACGGGGAATAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGCGCAGAATAGTAAAGCGCGTTTGTGGATTCGCCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGCAAACTCGTTGGCGAAGCGGTGAGGGCGAAAATCT > NZ_CP009273/901653‑901823 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |