Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	979,260	T→C	I52T (ATC→ACC)	gloC →	hydroxyacylglutathione hydrolase GloC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	979,260	0	T	C	100.0%	71.8 / NA	21	I52T (ATC→ACC)	gloC	hydroxyacylglutathione hydrolase GloC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/15);  total (6/15)

GTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGATCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGG  >  NZ_CP009273/979176‑979323                                                                                     |                                                                gTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCctgc                                                            >  2:181428/1‑90 (MQ=255)   cTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCctgctg                                                          <  2:676497/90‑1 (MQ=255)   cTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCctgctg                                                          <  1:268853/90‑1 (MQ=255)   cTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCctgctg                                                          <  1:776971/90‑1 (MQ=255)   cTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCctgctg                                                          <  2:732810/90‑1 (MQ=255)   cTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCctgctg                                                          <  1:655404/90‑1 (MQ=255)      gCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACg                                                       <  2:573385/90‑1 (MQ=255)         gCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCAt                                                    <  1:915115/90‑1 (MQ=255)          cACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATg                                                   <  1:506834/90‑1 (MQ=255)                tCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATc                                             <  2:900332/90‑1 (MQ=255)                tCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATc                                             >  2:187370/1‑90 (MQ=255)                 cGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCt                                            <  1:356678/90‑1 (MQ=255)                      cTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGAcc                                       <  1:387073/90‑1 (MQ=255)                      cTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGAcc                                       <  1:907188/90‑1 (MQ=255)                        ggcggcGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCAc                                     >  2:73274/1‑90 (MQ=255)                        ggcggcGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCAc                                     >  1:164330/1‑90 (MQ=255)                             cgATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTgg                                >  1:213863/1‑90 (MQ=255)                                            cAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACt                 <  1:693443/90‑1 (MQ=255)                                               aCAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGgc              <  1:857868/90‑1 (MQ=255)                                                          atgaCAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACg   >  2:512481/1‑90 (MQ=255)                                                           tgaCAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACgg  <  1:413021/90‑1 (MQ=255)                                                                                     |                                                                GTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGATCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGG  >  NZ_CP009273/979176‑979323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑