Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,320,565	G→A	Q521Q (CAG→CAA)	yciQ →	DUF2207 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,320,565	0	G	A	100.0%	87.9 / NA	23	Q521Q (CAG→CAA)	yciQ	DUF2207 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/13);  total (10/13)

GCGGGTTATTTTACCGCTGTTTGCCTGACCGGTTATCTCACTGGCATGGGGTATATTTTTCTGCCAAAGTTTACCCAAACTGGACAGCAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGGCAGCAACACACAGTGGACGT  >  NZ_CP009273/1320479‑1320637                                                                                       |                                                                         gCGGGTTATTTTACCGCTGTTTGCCTGACCGGTTATCTCACTGGCATGGGGTATATTTTTCTGCCAAAGTTTACCCAAACTGGAcaacaa                                                                       >  2:447776/1‑90 (MQ=255) gCGGGTTATTTTACCGCTGTTTGCCTGACCGGTTATCTCACTGGCATGGGGTATATTTTTCTGCCAAAGTTTACCCAAACTGGAcaacaa                                                                       >  1:887544/1‑90 (MQ=255)           ttACCGCTGTTTGCCTGACCGGTTATCTCACTGGCATGGGGTATATTTTTCTGCCAAAGTTTGCCCAAACTGGACAACAACGTTATGccc                                                             <  1:525464/90‑1 (MQ=255)           ttACCGCTGTTTGCCTGACCGGTTATCTCACTGGCATGGGGTATATTTTTCTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGccc                                                             <  2:700252/90‑1 (MQ=255)             aCCGCTGTTTGCCTGACCGGTTATCTCACTGGCATGGGGTATATTTTTCTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGCCCAc                                                           <  1:789952/90‑1 (MQ=255)                                gTTATCTCACTGGCATGGGGTATATTTTTCTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGCCCACGGTGAAGCTATCGTTAACt                                        <  1:27661/90‑1 (MQ=255)                                               tGGGGTATATTTTTCTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAag                         <  2:887544/90‑1 (MQ=255)                                                   gTATATTTTTCTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGgc                     <  2:538466/90‑1 (MQ=255)                                                     atatTTTTCTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGCCCACGGTGAAGc                                                   >  2:843633/1‑58 (MQ=255)                                                     atatTTTTCTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGCCCACGGTGAAGc                                                   <  1:843633/58‑1 (MQ=255)                                                     atatTTTTCTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGCCCACGGTGAAGCTa                                                 >  2:819281/1‑60 (MQ=255)                                                     atatTTTTCTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGCCCACGGTGAAGCTa                                                 <  1:819281/60‑1 (MQ=255)                                                     atatTTTTCTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGgcag                   >  1:353367/1‑90 (MQ=255)                                                         ttttCTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGGCAGCAac               <  1:447776/90‑1 (MQ=255)                                                           ttCTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGGCAGCAacac             >  2:652008/1‑90 (MQ=255)                                                           ttCTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGGCAGCAacac             >  1:146134/1‑90 (MQ=255)                                                           ttCTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGGCAGCAacac             >  2:81566/1‑90 (MQ=255)                                                           ttCTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGGCAGCAacac             >  1:58963/1‑90 (MQ=255)                                                            tCTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGCCCACGGTGa                                                      >  1:514221/1‑48 (MQ=255)                                                            tCTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGCCCACGGTGa                                                      <  2:514221/48‑1 (MQ=255)                                                             cTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAag                         <  2:425592/76‑1 (MQ=255)                                                             cTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAag                         >  1:425592/1‑76 (MQ=255)                                                                      tttACCCAAACTGGACAACAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGGCAGCAACACACAGTGGACGt  <  2:353367/90‑1 (MQ=255)                                                                         aCCCAAACTGGACAACAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGgc                     >  2:767829/1‑68 (MQ=255)                                                                         aCCCAAACTGGACAACAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGgc                     <  1:767829/68‑1 (MQ=255)                                                                                       |                                                                         GCGGGTTATTTTACCGCTGTTTGCCTGACCGGTTATCTCACTGGCATGGGGTATATTTTTCTGCCAAAGTTTACCCAAACTGGACAGCAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGGCAGCAACACACAGTGGACGT  >  NZ_CP009273/1320479‑1320637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑