Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,325,886	T→C	G194G (GGT→GGC)	topA →	type I DNA topoisomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,325,886	0	T	C	100.0%	77.7 / NA	23	G194G (GGT→GGC)	topA	type I DNA topoisomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/13);  total (10/13)

GCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAG  >  NZ_CP009273/1325799‑1325956                                                                                        |                                                                       gcagGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTgccggccg                                                                      <  1:506594/90‑1 (MQ=255)  cagGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGCCgt                                                                     <  2:133061/90‑1 (MQ=255)         gTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGCCGTGTGCAGt                                                              >  1:260988/1‑90 (MQ=255)          tCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGCCGTGTGCAGTc                                                             <  2:687230/90‑1 (MQ=255)                  tGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCgg                                                     <  2:377998/90‑1 (MQ=255)                    gACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCGGtt                                                   <  1:391172/90‑1 (MQ=255)                     aCCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCGGTTc                                                  <  2:644475/90‑1 (MQ=255)                     aCCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCGGTTc                                                  <  2:185241/90‑1 (MQ=255)                      ccGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCGGTTCg                                                 >  2:148936/1‑90 (MQ=255)                      ccGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCGGTTCg                                                 >  2:627779/1‑90 (MQ=255)                      ccGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCGGTTCg                                                 >  2:273559/1‑90 (MQ=255)                                       tGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCgagcgtg                                <  2:74988/90‑1 (MQ=255)                                       tGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCgagcgtg                                >  2:33301/1‑90 (MQ=255)                                           ttCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCGGTTCGCCt                                              <  1:285539/72‑1 (MQ=255)                                           ttCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCGGTTCGCCt                                              >  2:285539/1‑72 (MQ=255)                                           ttCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCgagcgtgagcg                            >  2:339839/1‑90 (MQ=255)                                           ttCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCgagcgtgagcg                            >  1:838932/1‑90 (MQ=255)                                            tCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCgagcgtgagcgt                           <  2:190319/90‑1 (MQ=255)                                              gccgcTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCGGTTc                                                  >  2:364727/1‑65 (MQ=255)                                              gccgcTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCGGTTc                                                  <  1:364727/65‑1 (MQ=255)                                              gccgcTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAgcgtgagcgtga                         >  2:916829/1‑90 (MQ=255)                                                           aaaaaGATCGCTCGTGGCCTGTCTGCCGGCTGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTc            <  2:820922/90‑1 (MQ=255)                                                                gATCGCTCGTGGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGcc       <  1:657180/90‑1 (MQ=255)                                                                     cTCGTGGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAag  <  2:271810/90‑1 (MQ=255)                                                                                        |                                                                       GCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAG  >  NZ_CP009273/1325799‑1325956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑