Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,331,016	A→G	Y310C (TAT→TGT)	acnA →	aconitate hydratase AcnA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,331,016	0	A	G	100.0%	52.4 / NA	16	Y310C (TAT→TGT)	acnA	aconitate hydratase AcnA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/8);  total (8/8)

GGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATC  >  NZ_CP009273/1330930‑1331093                                                                                       |                                                                              ggggAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTgg                                                                            >  2:330111/1‑90 (MQ=255)   ggAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCTAATATGTCGCCAGAATgtggtg                                                                          >  2:136508/1‑90 (MQ=255)    gAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGc                                                                         <  2:418778/90‑1 (MQ=255)       aTTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCAc                                                                      <  1:330111/90‑1 (MQ=255)                          gATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATc                                                   >  1:200261/1‑90 (MQ=255)                          gATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATc                                                   >  1:664961/1‑90 (MQ=255)                            tGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTCGCTTCTTCCCAATCGa                                                 <  2:459728/90‑1 (MQ=255)                                  ggATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGt                                           <  2:16867/90‑1 (MQ=255)                                       cACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGc                                              >  1:295301/1‑82 (MQ=255)                                       cACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGc                                              <  2:295301/82‑1 (MQ=255)                                       cACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAAccc                                      >  2:6807/1‑90 (MQ=255)                                        aCTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCt                                     <  1:189047/90‑1 (MQ=255)                                              gTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTa                               >  1:616537/1‑90 (MQ=255)                                                                         tatGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGa    >  1:181362/1‑90 (MQ=255)                                                                           tGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATc  <  2:616537/90‑1 (MQ=255)                                                                              cGCCAGAATGTGGTGCCCCCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGgcg             <  2:90253/76‑1 (MQ=255)                                                                              cGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGgcg             >  1:90253/1‑76 (MQ=255)                                                                                       |                                                                              GGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATC  >  NZ_CP009273/1330930‑1331093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑