Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,431,536	T→C	E1169G (GAA→GGA)	nifJ ←	pyruvate:ferredoxin (flavodoxin) oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,431,536	0	T	C	100.0%	69.0 / NA	20	E1169G (GAA→GGA)	nifJ	pyruvate:ferredoxin (flavodoxin) oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/9);  total (11/9)

CAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCC  >  NZ_CP009273/1431457‑1431616                                                                                |                                                                                 cAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTCCCGCTTTTcc                                                                        >  2:696618/1‑90 (MQ=255) cAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTCCCGCTTTTcc                                                                        >  1:912268/1‑90 (MQ=255)  aaGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTCCCg                                                                               <  2:244620/82‑1 (MQ=255)  aaGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTCCCg                                                                               >  1:244620/1‑82 (MQ=255)  aaGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCg                                                                       >  1:32586/1‑90 (MQ=255)             gtcgtcCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGc                                                            >  2:854138/1‑90 (MQ=255)             gtcgtcCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGc                                                            >  2:613044/1‑90 (MQ=255)                gtcCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAg                                                         <  1:300985/90‑1 (MQ=255)                 tcCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAgg                                                        <  2:846638/90‑1 (MQ=255)                  ccGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGa                                                       <  2:291231/90‑1 (MQ=255)                  ccGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGa                                                       <  2:299681/90‑1 (MQ=255)                                                    ccAGAGCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATc                     <  2:808663/90‑1 (MQ=255)                                                      agagCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCtt                   >  2:722203/1‑90 (MQ=255)                                                       gagCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCttt                  >  2:296444/1‑90 (MQ=255)                                                       gagCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCttt                  >  1:99752/1‑90 (MQ=255)                                                       gagCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCttt                  >  1:517121/1‑90 (MQ=255)                                                         gCATTTAATCGGTGTTGCTTTTTCCAGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTcc                <  1:345852/90‑1 (MQ=255)                                                                    gtgtTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCt     >  1:442743/1‑90 (MQ=255)                                                                       ttGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGcc  <  2:180944/90‑1 (MQ=255)                                                                       ttGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGcc  <  2:99752/90‑1 (MQ=255)                                                                                |                                                                                 CAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCC  >  NZ_CP009273/1431457‑1431616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑