Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,502,076	A→G	N347D (AAC→GAC)	rlhA →	23S rRNA 5‑hydroxycytidine C2501 synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,502,076	0	A	G	100.0%	75.7 / NA	22	N347D (AAC→GAC)	rlhA	23S rRNA 5‑hydroxycytidine C2501 synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/11);  total (11/11)

TGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAGTACCGCGTCT  >  NZ_CP009273/1502011‑1502154                                                                  |                                                                               tGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCAATGGCCTGGACGTGTTGATTAAACGTGAAgttg                                                        >  1:531443/1‑88 (MQ=255)  gAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAgtcgt                                                       >  2:785904/1‑90 (MQ=255)       tGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGtt                                                  >  2:795796/1‑90 (MQ=255)       tGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGtt                                                  >  2:325951/1‑90 (MQ=255)         gCGAAAGATCATCTCGATGTGGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGtttt                                                <  2:518311/90‑1 (MQ=255)          cGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGtttcc                                               <  1:795796/90‑3 (MQ=255)          cGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTc                                               <  2:216400/90‑1 (MQ=255)                  catcTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAAt                                       >  1:840241/1‑90 (MQ=255)                    tctcGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATAc                                     <  2:264755/90‑1 (MQ=255)                            ttcccGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCgaga                             <  2:568337/87‑1 (MQ=255)                                   tACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCgg                      >  1:586903/1‑90 (MQ=255)                                    cccGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGga                     <  2:4732/89‑1 (MQ=255)                                      cGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGgaga                   <  1:325951/90‑1 (MQ=255)                                      cGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGgaga                   <  2:586903/90‑1 (MQ=255)                                        aGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGaaa                 >  1:328544/1‑90 (MQ=255)                                        aGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGCCGAGAAAACCGGAGaaa                 >  2:908430/1‑90 (MQ=255)                                           cACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATc              >  2:689319/1‑90 (MQ=255)                                                       gCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAGTACCGCGTCt  <  1:103324/90‑1 (MQ=255)                                                         gATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAGTACCGCGTCt  <  1:711951/88‑1 (MQ=255)                                                         gATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAGTACCGCGTCt  >  2:711951/1‑88 (MQ=255)                                                             gCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAGTACCGCGTCt  <  1:913497/84‑1 (MQ=255)                                                             gCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAGTACCGCGTCt  >  2:913497/1‑84 (MQ=255)                                                                  |                                                                               TGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAGTACCGCGTCT  >  NZ_CP009273/1502011‑1502154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑