Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,503,965	T→C	D67D (GAT→GAC)	hicB →	type II toxin‑antitoxin system antitoxin HicB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,503,965	0	T	C	100.0%	87.0 / NA	25	D67D (GAT→GAC)	hicB	type II toxin‑antitoxin system antitoxin HicB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/11);  total (14/11)

TGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAG  >  NZ_CP009273/1503887‑1504049                                                                               |                                                                                     tGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGa                                                                           >  2:728338/1‑90 (MQ=255)  gCTTTACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGaa                                                                          >  2:82771/1‑90 (MQ=255)    tttACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGt                                                                        <  1:338712/90‑1 (MQ=255)    tttACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGt                                                                        <  1:728338/90‑1 (MQ=255)      tACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTAc                                                                      >  2:84801/1‑90 (MQ=255)       aCTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTAcc                                                                     >  1:145056/1‑90 (MQ=255)            ccGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGa                                                                >  1:132544/1‑90 (MQ=255)                 tttGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTc                                                           <  1:620722/90‑1 (MQ=255)                 tttGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTc                                                           <  1:745429/90‑1 (MQ=255)                  ttGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCg                                                          <  2:145056/90‑1 (MQ=255)                                 aaGATAACGAGCTTATCCGTTTGCCTTCGCCATTAAATAGTCACGACCACTTTATGGAAGTACCTTTGAGCGTCGCCCCTAAGGTATTGc                                           <  2:452639/90‑1 (MQ=255)                                             ttATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCtt                               <  1:750155/90‑1 (MQ=255)                                             ttATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCtt                               >  2:68709/1‑90 (MQ=255)                                                  ccTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTa                          <  1:813111/90‑1 (MQ=255)                                                    tttACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACa                        >  1:10327/1‑90 (MQ=255)                                                     ttACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAg                       >  1:104909/1‑90 (MQ=255)                                                     ttACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAg                       >  2:159265/1‑90 (MQ=255)                                                     ttACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAg                       >  1:881713/1‑90 (MQ=255)                                                     ttACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAg                       >  1:833927/1‑90 (MQ=255)                                                          ttCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAg                                                  >  2:410528/1‑58 (MQ=255)                                                          ttCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAg                                                  <  1:410528/58‑1 (MQ=255)                                                          ttCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGa                  >  1:825882/1‑90 (MQ=255)                                                           tCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGaa                 <  1:24997/90‑1 (MQ=255)                                                                aTTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGAAATCAc            <  2:825882/90‑1 (MQ=255)                                                                          cACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGGAATCACTCAGCAagag  >  2:22734/1‑90 (MQ=255)                                                                               |                                                                                     TGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAG  >  NZ_CP009273/1503887‑1504049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑