Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,524,815	T→C	intergenic (‑54/‑28)	BW25113_RS25600 ← / → BW25113_RS07655	protein YncO/ISAs1 family transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,524,815	0	T	C	100.0%	79.1 / NA	24	intergenic (‑54/‑28)	BW25113_RS25600/BW25113_RS07655	protein YncO/ISAs1 family transposase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/12);  total (12/12)

GGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGACAAC  >  NZ_CP009273/1524729‑1524897                                                                                       |                                                                                   gggcaAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGaacaac                                                                                 >  1:144237/1‑90 (MQ=255) gggcaAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGaacaac                                                                                 >  1:291328/1‑90 (MQ=255) gggcaAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGaacaac                                                                                 >  1:731637/1‑90 (MQ=255) gggcaAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGaacaac                                                                                 >  1:726737/1‑90 (MQ=255)      aGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTaaaa                                                                            >  1:773866/1‑90 (MQ=255)      aGATCATGAAAATTGTGAGGTAAATCACGATTTTCATCTTTGCTTTAAGGGCTACAGGGGATAAAAATTTAACCTTTGGAACAACTaaaa                                                                            >  2:138292/1‑90 (MQ=255)           aTGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATa                                                                       >  2:227245/1‑90 (MQ=255)                    gtgATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGAc                                                              <  2:291328/90‑1 (MQ=255)                              tCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAg                                                    <  2:790878/90‑1 (MQ=255)                               cACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGt                                                   <  1:243954/90‑1 (MQ=255)                                          tCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGttt                                        <  2:731637/90‑1 (MQ=255)                                            tttGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGc                                      <  1:879798/90‑1 (MQ=255)                                             ttGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCaa                                             <  1:539732/82‑1 (MQ=255)                                             ttGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCaa                                             >  2:539732/1‑82 (MQ=255)                                              tGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCtt                                    <  2:69511/90‑1 (MQ=255)                                                         cTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGt                                                       >  1:558177/1‑60 (MQ=255)                                                         cTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGt                                                       <  2:558177/60‑1 (MQ=255)                                                         cTACAGGTGATCAAAATTTAACCTTTGAAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTc                         >  2:112291/1‑90 (MQ=255)                                                          tACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCa                        >  2:94715/1‑90 (MQ=255)                                                                tGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGAcc                  <  1:429638/90‑1 (MQ=255)                                                                 gATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGAccc                 >  2:53437/1‑90 (MQ=255)                                                                 gATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGAccc                 >  1:475729/1‑90 (MQ=255)                                                                  aTCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGAcc                  <  2:290652/88‑1 (MQ=255)                                                                  aTCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGAcc                  >  1:290652/1‑88 (MQ=255)                                                                   tccaaaTTTAACCTTTGGAACATCAAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCATAATTTGCTTGATTATAATTCACTCAccccc               <  2:743091/87‑2 (MQ=255)                                                                           tAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACg       >  2:629533/1‑90 (MQ=255)                                                                                tttGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGacaac  >  1:376571/1‑90 (MQ=255)                                                                                tttGAAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGacaac  <  1:112291/90‑1 (MQ=255)                                                                                       |                                                                                   GGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGACAAC  >  NZ_CP009273/1524729‑1524897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑