Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,531,275	G→A	L98L (CTG→TTG)	narW ←	nitrate reductase molybdenum cofactor assembly chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,531,275	0	G	A	100.0%	120.4 / NA	32	L98L (CTG→TTG)	narW	nitrate reductase molybdenum cofactor assembly chaperone
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/8);  total (24/8)

TCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAGCTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAA  >  NZ_CP009273/1531196‑1531359                                                                                |                                                                                     tcttTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCg                                                                            <  1:723065/90‑1 (MQ=255)   tttCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGAc                                                                          <  2:899872/90‑1 (MQ=255)      cGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCtt                                                                       >  1:63215/1‑90 (MQ=255)       gCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCttt                                                                      >  1:433172/1‑90 (MQ=255)           gATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCAt                                                                  >  1:192679/1‑90 (MQ=255)           gATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCAt                                                                  >  1:774311/1‑90 (MQ=255)           gATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCAt                                                                  >  2:320377/1‑90 (MQ=255)                       gTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCcagca                                                      >  1:397497/1‑90 (MQ=255)                           gCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTc                                                  <  1:441052/90‑1 (MQ=255)                                 aTACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGGCCTTTTCATACTCCGCCAGCAGGTCCACCAt                                            <  1:205133/90‑1 (MQ=255)                                 aTACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCAt                                            <  1:562251/90‑1 (MQ=255)                                    cTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGc                                         >  2:685962/1‑90 (MQ=255)                                     tCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGcc                                        >  2:456686/1‑90 (MQ=255)                                     tCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGcc                                        <  1:685962/90‑1 (MQ=255)                                      ccAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCt                                       >  2:697986/1‑90 (MQ=255)                                            aCAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCcgc                                 >  2:574490/1‑90 (MQ=255)                                             cAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCcgcg                                >  1:150009/1‑90 (MQ=255)                                              aGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGa                               >  1:154165/1‑90 (MQ=255)                                              aGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGa                               >  1:712144/1‑90 (MQ=255)                                              aGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGa                               >  1:434943/1‑90 (MQ=255)                                                  gTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATcgc                           >  2:592138/1‑90 (MQ=255)                                                    aGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCgg                         >  2:477176/1‑90 (MQ=255)                                                           cGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCgg                  >  2:772097/1‑90 (MQ=255)                                                            gggCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGc                 >  2:848781/1‑90 (MQ=255)                                                             ggCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCa                <  1:456686/90‑1 (MQ=255)                                                                 gTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGaa            >  1:458682/1‑90 (MQ=255)                                                                 gTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGaa            >  1:190719/1‑90 (MQ=255)                                                                   tCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACa          >  1:535907/1‑90 (MQ=255)                                                                           aTCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAAc           <  1:9859/81‑1 (MQ=255)                                                                           aTCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAAc           >  2:9859/1‑81 (MQ=255)                                                                           aTCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGaa  >  2:531644/1‑90 (MQ=255)                                                                           aTCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGaa  >  1:201947/1‑90 (MQ=255)                                                                                |                                                                                     TCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAGCTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAA  >  NZ_CP009273/1531196‑1531359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑