Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,558,439	A→G	Y518H (TAT→CAT)	dosP ←	oxygen‑sensing cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,558,439	0	A	G	100.0%	69.3 / NA	20	Y518H (TAT→CAT)	dosP	oxygen‑sensing cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/10);  total (10/10)

CTGCGCCTAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGATGCCAATACTC  >  NZ_CP009273/1558352‑1558512                                                                                        |                                                                          cTGCGCCTAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGAt                                                                         >  2:352176/1‑90 (MQ=255)  tGCGCCAAAAACCAACCCCTTTTTTCCCTTTTCTTTCATCCCGGGCCTGAAAAACTCCCAGCCGTTACCGCCATTCTTGCGAATATGATc                                                                        <  2:291418/90‑1 (MQ=255)   gcgcCTAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATcc                                                                       >  2:581832/1‑90 (MQ=255)         aaacCCAAACGCTTTTTTCCCTTTTCTTTCACCGCGGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCcattgca                                                                 <  1:294401/90‑1 (MQ=255)            aCCAACCGCTTTTTTCCCTTTTCTTTCACCGCGGGGCTGAAGACCTCCCAGCCGTTACCGCCATTCTTGCGAATATGATCcattgcattg                                                              <  2:920021/90‑1 (MQ=255)              cAAACGCTCTTTTCCCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATtgtg                                                            <  1:391833/90‑1 (MQ=255)                      ctTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTgg                                                    >  2:886636/1‑90 (MQ=255)                              aTTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAg                                            >  1:699699/1‑90 (MQ=255)                              aTTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAg                                            >  2:389129/1‑90 (MQ=255)                              aTTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAg                                            >  2:789467/1‑90 (MQ=255)                                ttCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTa                                          >  1:524985/1‑90 (MQ=255)                                ttCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTa                                          >  2:602497/1‑90 (MQ=255)                                ttCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTa                                          >  1:11927/1‑90 (MQ=255)                                     tCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATcgc                                     <  1:433206/90‑1 (MQ=255)                                      cATCGCGGGGTTGAAGACCTCCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATCGTGAGCAGTGGAGAGCAAGTAATcgcg                                    <  2:451858/90‑1 (MQ=255)                                      cATCGCCGGGCGAAAGATCTGCCAGCGGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATcgcg                                    <  2:771386/90‑1 (MQ=255)                                             gggCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTa                             >  1:404097/1‑90 (MQ=255)                                                 acaagacCTCCCCGCCTTTCCCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCa                         <  2:71219/88‑1 (MQ=255)                                                       aCTCCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGt                   <  1:463368/90‑1 (MQ=255)                                                                        ccATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGATGCCAATACTc  <  2:447297/90‑1 (MQ=255)                                                                                        |                                                                          CTGCGCCTAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGATGCCAATACTC  >  NZ_CP009273/1558352‑1558512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑