Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,573,157	A→T	I148N (ATC→AAC)	yddA ←	ABC transporter ATP‑binding protein/permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,573,157	0	A	T	100.0%	116.1 / NA	31	I148N (ATC→AAC)	yddA	ABC transporter ATP‑binding protein/permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (12/19);  total (12/19)

CCGCGCTTTCCCAAAGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGAA  >  NZ_CP009273/1573071‑1573233                                                                                       |                                                                             ccGCGCTTTCCCAAAGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCa                                                                           <  1:96867/90‑1 (MQ=255)  cgcgCTTTCCCAAAGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAg                                                                          <  2:227597/90‑1 (MQ=255)   gcgcTTTCCCAAAGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGt                                                                         >  2:444836/1‑90 (MQ=255)   gcgcTTTCCCAAAGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGt                                                                         >  1:692811/1‑90 (MQ=255)        ttCCCAAAGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAagag                                                                    >  2:11115/1‑90 (MQ=255)         tCCCAAAGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAgaga                                                                   <  1:426052/90‑1 (MQ=255)                          aaaGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGaa                                                                  >  1:76198/1‑74 (MQ=255)                          aaaGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGaa                                                                  <  2:76198/74‑1 (MQ=255)                           aaGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGt                                                 <  2:692811/90‑1 (MQ=255)                           aaGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGt                                                 <  1:587397/90‑1 (MQ=255)                           aaGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGt                                                 <  2:797811/90‑1 (MQ=255)                               tGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGt                                             <  1:860665/90‑1 (MQ=255)                                gATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTc                                            >  2:775288/1‑90 (MQ=255)                                gATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTc                                            <  2:452311/90‑1 (MQ=255)                                 aTCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCa                                           >  1:204911/1‑90 (MQ=255)                                 aTCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCa                                           <  2:338344/90‑1 (MQ=255)                                 aTCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCa                                           >  1:115740/1‑90 (MQ=255)                                     gCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGt                                       >  1:98550/1‑90 (MQ=255)                                       aTGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTa                                     >  2:274314/1‑90 (MQ=255)                                            aaGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTc                                            <  1:28215/78‑1 (MQ=255)                                            aaGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTc                                            >  2:28215/1‑78 (MQ=255)                                                        gAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTc                    <  1:753557/90‑1 (MQ=255)                                                        gAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTc                    <  2:853807/90‑1 (MQ=255)                                                                  gAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATc          <  1:202531/90‑1 (MQ=255)                                                                  gAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATc          <  1:439081/90‑1 (MQ=255)                                                                       aCTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGt     <  1:342735/90‑1 (MQ=255)                                                                       aCTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGt     <  2:98550/90‑1 (MQ=255)                                                                         tGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGa   <  2:848310/90‑1 (MQ=255)                                                                         tGAGCGATTTTCTTTTCAGTAAGAGAATTTCTTCAGCGCTACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGa   <  2:636260/90‑1 (MQ=255)                                                                          gAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGaa  >  1:350340/1‑90 (MQ=255)                                                                          gAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGaa  >  1:332566/1‑90 (MQ=255)                                                                                       |                                                                             CCGCGCTTTCCCAAAGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGAA  >  NZ_CP009273/1573071‑1573233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑