Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,579,109	A→C	G545G (GGT→GGG)	ydeP ←	acid resistance putative oxidoreductase YdeP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,579,109	0	A	C	100.0%	147.5 / NA	41	G545G (GGT→GGG)	ydeP	acid resistance putative oxidoreductase YdeP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (21/20);  total (21/20)

CAGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACACCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACCGTTTTTTTGCA  >  NZ_CP009273/1579025‑1579194                                                                                     |                                                                                      caGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCcggc                                                                                  <  1:118299/90‑1 (MQ=255)  aGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCcggcg                                                                                 >  1:652388/1‑90 (MQ=255)     tACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGt                                                                              <  1:215628/90‑1 (MQ=255)         cccAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTa                                                                          >  2:99473/1‑90 (MQ=255)              gCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAAcacg                                                                     <  2:442383/90‑1 (MQ=255)                 aCCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAAcacgcca                                                                  >  2:705587/1‑90 (MQ=255)                    aCGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAAcacgccacgc                                                               <  1:82976/90‑1 (MQ=255)                      gCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGa                                                             <  1:99473/90‑1 (MQ=255)                      gCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGa                                                             <  1:521402/90‑1 (MQ=255)                      gCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGa                                                             >  1:265610/1‑90 (MQ=255)                      gCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGa                                                             <  1:789717/90‑1 (MQ=255)                                 gTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAatca                                                  >  1:306226/1‑90 (MQ=255)                                 gTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAatca                                                  >  2:315489/1‑90 (MQ=255)                                   gctgcCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAatcatc                                                <  1:755353/90‑1 (MQ=255)                                     tgcctgcGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGa                                              <  1:859162/90‑1 (MQ=255)                                     tgcctgcGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGa                                              <  1:442546/90‑1 (MQ=255)                                     tgcctgcGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGGTTTAACACGCCACGCGAGGCATGAATCATCGa                                              >  2:492090/1‑90 (MQ=255)                                        ctgcGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACa                                            <  1:240235/89‑1 (MQ=255)                                        ctgcGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACa                                            >  2:240235/1‑89 (MQ=255)                                          gcgcGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTg                                         <  2:265610/90‑1 (MQ=255)                                            gcgATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGaa                                       <  1:750627/90‑1 (MQ=255)                                                         ccACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTa                          <  1:775145/90‑1 (MQ=255)                                                           aCTGCACACTCTGATTTCAGCATTCCCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTAcc                        <  2:732315/90‑1 (MQ=255)                                                           aCTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTAcc                        >  2:383505/1‑90 (MQ=255)                                                              gCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGcc                     >  2:315100/1‑90 (MQ=255)                                                                  acTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGa                                              <  1:607981/61‑1 (MQ=255)                                                                  acTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGa                                              >  2:607981/1‑61 (MQ=255)                                                                   ctctGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTgcgc                <  1:483745/90‑1 (MQ=255)                                                                     ctGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCAc              >  1:801709/1‑90 (MQ=255)                                                                      tGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCAcc             >  1:234990/1‑90 (MQ=255)                                                                        aTTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCAcc             <  2:666820/88‑1 (MQ=255)                                                                        aTTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCAcc             >  1:666820/1‑88 (MQ=255)                                                                            cAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTAcc                        <  2:450281/73‑1 (MQ=255)                                                                            cAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTAcc                        >  1:450281/1‑73 (MQ=255)                                                                             aGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTAcc                        <  2:491876/72‑1 (MQ=255)                                                                             aGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTAcc                        >  1:491876/1‑72 (MQ=255)                                                                             aGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACCGtttttt      >  1:848240/1‑90 (MQ=255)                                                                             aGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACCGtttttt      >  1:6290/1‑90 (MQ=255)                                                                             aGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACCGtttttt      >  2:75991/1‑90 (MQ=255)                                                                               cATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACCGTTTTTTTg    >  2:750323/1‑90 (MQ=255)                                                                                 ttACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACCGTTTTTTTGCa  >  2:918631/1‑90 (MQ=255)                                                                                     |                                                                                      CAGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACACCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACCGTTTTTTTGCA  >  NZ_CP009273/1579025‑1579194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑