| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,615,188 | A→C | S69R (AGT→AGG) | eamA ← | O‑acetylserine/cysteine exporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,615,188 | 0 | A | C | 92.3% | 82.4 / ‑0.2 | 26 | S69R (AGT→AGG) | eamA | O‑acetylserine/cysteine exporter |
| Reads supporting (aligned to +/- strand): ref base A (0/2); new base C (8/16); total (8/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.97e-01 | |||||||||||
AAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGAC > NZ_CP009273/1615101‑1615268 | aaaCGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAACCt > 2:909509/1‑90 (MQ=255) aCGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAACCTGa < 2:339882/90‑1 (MQ=255) aCGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAACCTGa < 1:95598/90‑1 (MQ=255) aCGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAACCTGa < 1:906206/90‑1 (MQ=255) aCGCCTGAGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAACCTGa < 1:67471/90‑1 (MQ=255) tgtgcctgtAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAACCTGATGGtt < 2:365748/90‑1 (MQ=255) tgtgcctgtAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAACCTGATGGtt < 1:109644/90‑1 (MQ=255) tgtgcctgtAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAACCTGATGGtt < 1:475989/90‑1 (MQ=255) gtgcctgtAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTa < 2:771868/90‑1 (MQ=255) tgcctgtAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAACCTGATGGTTaa < 1:909509/90‑1 (MQ=255) tgcctgtAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAACCTGATGGTTaa < 2:645692/90‑1 (MQ=255) aaGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAgcagca < 2:303535/90‑1 (MQ=255) aGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAACCTGATGGTTAATCCATACCCCAGCAGCaa < 1:337373/90‑1 (MQ=255) aGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAACCTGATGGTTAATCCATACCCCAGCAGCaa > 1:195882/1‑90 (MQ=255) gCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAACCTGa > 1:70346/1‑64 (MQ=255) gCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAACCTGa < 2:70346/64‑1 (MQ=255) ccAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAACCTGATGGTTAATCCATACCCCAGCAGCAAAt < 1:526444/90‑1 (MQ=255) gTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAACCTGATGGTTAATCCATACCCCAGCAGCAAATTCa > 2:94712/1‑90 (MQ=255) agcagGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAACCTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTgg < 2:724465/90‑1 (MQ=255) aGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAACCTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTg > 2:700759/1‑90 (MQ=255) cacaAAAAAGAAAAGCAAACTGCGCAAACGTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACaaaaa > 1:732855/1‑90 (MQ=255) cacaAAAAAGAAAAGCAAACTGCGCAAACCTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACaaaaa > 2:183986/1‑90 (MQ=255) aCTGCGCAAACCTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGa > 1:838462/1‑90 (MQ=255) cTGCGCAAGCCGGAAGGTTAATCCATCCCCCAGCAGCAAATTCAGTCGTCCTTTCGGTCGTGCGACAAAAAAGGTAGCCGGAAAAGCGAc < 2:13266/90‑1 (MQ=255) cTGCGCAAACCTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGAc < 2:195882/90‑1 (MQ=255) cTGCGCAAACCTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGAc < 2:838462/90‑1 (MQ=255) | AAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGAC > NZ_CP009273/1615101‑1615268 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |