Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 1,662,649 T→G H58P (CAC→CCC)  mlc ← DNA‑binding transcriptional repressor Mlc

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,662,6490TG100.0% 49.6 / NA 15H58P (CAC→CCC) mlcDNA‑binding transcriptional repressor Mlc
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (6/9);  total (6/9)

GTGCCAGGCTTCAGTTTCAACCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGAC  >  NZ_CP009273/1662564‑1662731
                                                                                     |                                                                                  
gTGCCAGGCTTCAGTTTCAACCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGc                                                                                >  2:703511/1‑90 (MQ=255)
  gCCAGGCTTCAGTTTCAACCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGCtt                                                                              >  1:352592/1‑90 (MQ=255)
     aGGCTTCAGTTTCAACCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGa                                                                           >  1:497540/1‑90 (MQ=255)
                tCAACCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACgg                                                                <  1:359496/90‑1 (MQ=255)
                       ccaGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACGGACAAttt                                                         >  1:900915/1‑90 (MQ=255)
                                  gccgGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTg                                              <  1:703511/90‑1 (MQ=255)
                                     ggACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCa                                           <  1:520046/90‑1 (MQ=255)
                                       aCGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAgg                                         >  1:670352/1‑90 (MQ=255)
                                         ggCCACAGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAg                                       <  2:557464/90‑1 (MQ=255)
                                                       gCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGa                         <  1:384782/90‑1 (MQ=255)
                                                          tcttTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCGTCTCACGGACAATTTTAGTGATGCTGGCAGGAGCCAGTTGCGCCAGACgg                      >  1:526617/1‑90 (MQ=255)
                                                                  ttCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATc              <  2:497540/90‑1 (MQ=255)
                                                                         tctTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATAcgc       <  1:820373/90‑1 (MQ=255)
                                                                         tctTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATAcgc       <  2:876042/90‑1 (MQ=255)
                                                                              cACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGAc  <  2:381744/90‑1 (MQ=255)
                                                                                     |                                                                                  
GTGCCAGGCTTCAGTTTCAACCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGAC  >  NZ_CP009273/1662564‑1662731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: