Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,717,498	G→C	G374A (GGC→GCC)	ydhK →	FUSC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,717,498	0	G	C	100.0%	63.0 / NA	19	G374A (GGC→GCC)	ydhK	FUSC family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (8/11);  total (8/11)

CCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCG  >  NZ_CP009273/1717410‑1717584                                                                                         |                                                                                       ccAAGGACATCCAGCCTGGCTCGTCATACCGGTAACGCCGAAGCTATGTGGAGCCGGCTGCGTACATTTTGTACGTTGATGATGATTGgc                                                                                       >  2:507471/1‑90 (MQ=255)     ggACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCAt                                                                                   <  1:260381/90‑1 (MQ=255)        cATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGa                                                                                >  1:58765/1‑90 (MQ=255)                           tACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGa                                                             <  2:385407/90‑1 (MQ=255)                                       cGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCaa                                                 <  1:485340/90‑1 (MQ=255)                                        gAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAAt                                                <  2:628950/90‑1 (MQ=255)                                        gAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAAt                                                <  2:568633/90‑1 (MQ=255)                                         aaGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATg                                               >  1:493247/1‑90 (MQ=255)                                              aTGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTa                                          <  2:431434/90‑1 (MQ=255)                                                   gAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCt                                     >  1:666953/1‑90 (MQ=255)                                                   gAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCt                                     >  2:171452/1‑90 (MQ=255)                                                      cGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGgc                                  <  1:383283/90‑1 (MQ=255)                                                        ggCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCTCAATGGGATGCCGGTGCCAATGCATTAACGCTGgcag                                >  1:548378/1‑90 (MQ=255)                                                            gCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGGATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAAt                            >  2:422342/1‑90 (MQ=255)                                                             cGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAAtt                           <  2:578461/90‑1 (MQ=255)                                                                  aTTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTg                      <  2:268685/90‑1 (MQ=255)                                                                    tttGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCg                    <  2:493247/90‑1 (MQ=255)                                                                      tGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGta                  >  1:785039/1‑90 (MQ=255)                                                                                  atgatTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTccgcc      <  1:438367/90‑1 (MQ=255)                                                                                      ttGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCg  >  2:631073/1‑90 (MQ=255)                                                                                         |                                                                                       CCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCG  >  NZ_CP009273/1717410‑1717584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑