Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,803,201	A→G	V97A (GTC→GCC)	yniB ←	YniB family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,803,201	0	A	G	100.0%	112.9 / NA	32	V97A (GTC→GCC)	yniB	YniB family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/19);  total (13/19)

GAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTGGGCGTCGGGGAGTTATACC  >  NZ_CP009273/1803122‑1803276                                                                                |                                                                            gAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAg                                                                   >  1:755194/1‑90 (MQ=255)  aGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAgg                                                                  >  2:376052/1‑90 (MQ=255)  aGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAgg                                                                  >  2:569327/1‑90 (MQ=255)           tCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATc                                                         >  2:616264/1‑90 (MQ=255)               cacCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCAccc                                                     <  2:45565/90‑1 (MQ=255)                acCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCa                                                    <  1:616264/90‑1 (MQ=255)                   ttCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGaa                                                 >  1:354990/1‑90 (MQ=255)                   ttCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGaa                                                 >  1:459776/1‑90 (MQ=255)                   ttCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGaa                                                 >  1:472321/1‑90 (MQ=255)                   ttCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGaa                                                 >  1:770435/1‑90 (MQ=255)                        gCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATaa                                            >  2:279466/1‑90 (MQ=255)                        gCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATaa                                            <  1:684086/90‑1 (MQ=255)                           agaagCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGt                                         >  1:656011/1‑90 (MQ=255)                                  cgcCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGcc                                  <  1:577212/90‑1 (MQ=255)                                  cgcCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGcc                                  <  2:745776/90‑1 (MQ=255)                                   gcCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGcca                                 <  2:472321/90‑1 (MQ=255)                                   gcCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGcca                                 <  2:755194/90‑1 (MQ=255)                                   gcCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGcca                                 <  1:99973/90‑1 (MQ=255)                                   gcCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGcca                                 <  1:873598/90‑1 (MQ=255)                                     cTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGccacc                               <  2:297527/90‑1 (MQ=255)                                              cATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGaaa                                                            >  2:422593/1‑52 (MQ=255)                                              cATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGaaa                                                            <  1:422593/52‑1 (MQ=255)                                                        ccGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTGGGCGTCggg            <  2:354990/90‑1 (MQ=255)                                                        ccGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTGGGCGTCggg            <  2:656011/90‑1 (MQ=255)                                                          ggAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCAccc                                                     <  2:377054/47‑1 (MQ=255)                                                          ggAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCAccc                                                     >  1:377054/1‑47 (MQ=255)                                                          ggAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCa                                                    <  1:330385/48‑1 (MQ=255)                                                          ggAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCa                                                    >  2:330385/1‑48 (MQ=255)                                                             aTCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTGGGCGTCGGGGAGtt       <  1:569327/90‑1 (MQ=255)                                                             aTCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTGGGCGTCGGGGAGtt       <  1:376052/90‑1 (MQ=255)                                                                  gCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTGGGCGTCGGGGAGTTATAcc  <  2:913386/90‑1 (MQ=255)                                                                  gCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTGGGCGTCGGGGAGTTATAcc  <  2:103022/90‑1 (MQ=255)                                                                                |                                                                            GAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTGGGCGTCGGGGAGTTATACC  >  NZ_CP009273/1803122‑1803276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑