Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,818,674	A→C	L174R (CTG→CGG)	ves ←	environmental stress‑induced protein Ves

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,818,674	0	A	C	100.0%	71.9 / NA	21	L174R (CTG→CGG)	ves	environmental stress‑induced protein Ves
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (12/9);  total (12/9)

TTGTAAGCCGCTGCTTTCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTC  >  NZ_CP009273/1818587‑1818738                                                                                        |                                                                 ttGTAAGCCGCTGCTTTCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGGACAACCGt                                                                >  1:704972/1‑90 (MQ=255) ttGTAAGCCGCTGCTTTCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACCGt                                                                >  1:571687/1‑90 (MQ=255)  tGTAAGCCGCTGCTTTCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACCGtt                                                               <  1:305767/90‑1 (MQ=255)    tAAGCCGCTGCTTTCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACCGTTTa                                                             >  1:743236/1‑90 (MQ=255)      aGCTGCTGCTTTCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACCGTTTAcc                                                           >  2:601085/1‑90 (MQ=255)          gctgctTTCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACCGTTTACCTTGc                                                       <  2:362454/90‑1 (MQ=255)                ttCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACCGTTTACCTTGCGGTTGt                                                 >  2:60977/1‑90 (MQ=255)                         cTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACCGTTTACCTTGCGGTTGTAAAAGCCGc                                        <  2:221830/90‑1 (MQ=255)                          tATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACCGTTTACCTTGCGGTTGTAAAAGCCGCa                                       >  1:364089/1‑90 (MQ=255)                               aaTTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACCGTTTACCTTGCGGTTGTAAAAGCCGCAGGgtg                                  >  2:566176/1‑90 (MQ=255)                               aaTTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACCGTTTACCTTGCGGTTGTAAAAGCCGCAGGgtg                                  >  2:503242/1‑90 (MQ=255)                                   aCTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACCGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGtgcc                              >  1:572382/1‑90 (MQ=255)                                    cTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACCGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGtgcct                             <  1:387099/90‑1 (MQ=255)                                           tGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACCGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCg                      >  2:870754/1‑90 (MQ=255)                                           tGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACCGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCg                      >  1:360217/1‑90 (MQ=255)                                                  gCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACCGTTTACCTTGCGGTTGTAAAAGCCGCAGGGGGTGCCTGCCATCGAACCAGc               >  1:745607/1‑90 (MQ=255)                                                      aTGCCCAGCGAGCCAGTTGATTTCACTGAACAACCGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCAcgc           <  1:304921/90‑1 (MQ=255)                                                      aTGACCAGCGAGCCAGTTGATTTCACTGAACAACCGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCAcgc           <  1:912640/90‑1 (MQ=255)                                                          ccAGCGAGCCAGTTGATTTCACTGAACAACCGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGccc       <  2:360217/90‑1 (MQ=255)                                                          ccAGCGAGCCAGTTGATTTCACTGAACAACCGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGccc       <  2:201809/90‑1 (MQ=255)                                                               gAGCCAGTTGATTTCACTGAACAACCGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTc  <  2:364089/90‑1 (MQ=255)                                                                                        |                                                                 TTGTAAGCCGCTGCTTTCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTC  >  NZ_CP009273/1818587‑1818738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑