Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,838,813	T→C	I106V (ATT→GTT)	ynjI ←	DUF1266 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,838,813	0	T	C	100.0%	157.0 / NA	44	I106V (ATT→GTT)	ynjI	DUF1266 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/19);  total (25/19)

CAGCGTTTCAGACCATTGCTTATGATAGAATCGATAAGGTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAATACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCCATCATAATTAATAGATAA  >  NZ_CP009273/1838727‑1838898                                                                                       |                                                                                      cAGCGTTTCAGACCATTGCTTATGATAGAATCGATAAGGTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACAcc                                                                                    >  2:191100/1‑90 (MQ=255)     gTTTCAGACCATTGCTTATGATAGAATCGATAAGGTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTa                                                                                <  2:810099/90‑1 (MQ=255)     gTTTCAGACCATTGCTTATGATAGAATCGATAAGGTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTa                                                                                <  2:10401/90‑1 (MQ=255)     gTTTCAGACCATTGCTTATGATAGAATCGATAAGGTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTa                                                                                <  1:904723/90‑1 (MQ=255)          aGACCATTGCTTATGATAGAATCGATAAGGTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTg                                                                           <  1:425729/90‑1 (MQ=255)                       tGATAGAATCGATAAGGTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAgt                                                              >  2:492539/1‑90 (MQ=255)                       tGATAGAATCGATAAGGTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAgt                                                              >  2:385446/1‑90 (MQ=255)                            gAATCGATAAGGTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTc                                                         >  1:125944/1‑90 (MQ=255)                            gAATCGATAAGGTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTc                                                         >  2:501605/1‑90 (MQ=255)                                   tAAGGTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCttttttt                                                  >  1:707930/1‑90 (MQ=255)                                      ggTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCt                                               >  1:712408/1‑90 (MQ=255)                                        tGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCttt                                             >  2:97261/1‑90 (MQ=255)                                             tCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAAc                                        <  2:508633/90‑1 (MQ=255)                                                 ttGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGc                                    >  2:571708/1‑90 (MQ=255)                                                   gTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGccc                                  >  2:200195/1‑90 (MQ=255)                                                      gcgcCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTg                                                                           >  1:137740/1‑46 (MQ=255)                                                      gcgcCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTg                                                                           <  2:137740/46‑1 (MQ=255)                                                      gcgcCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGaa                               <  1:385446/90‑1 (MQ=255)                                                      gcgcCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTACCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGaa                               >  2:38304/1‑90 (MQ=255)                                                        gcCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACa                             >  2:905230/1‑90 (MQ=255)                                                        gcCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACa                             >  1:385996/1‑90 (MQ=255)                                                        gcCTGTAACTTCTCGATACTGAGCGGCCCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTg                                                 <  2:232883/70‑1 (MQ=255)                                                        gcCTGTAACTTCTCGATACTGAGCGGCCCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTg                                                 >  1:232883/1‑70 (MQ=255)                                                          cTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAAt                           >  1:482090/1‑90 (MQ=255)                                                          cTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAAt                           >  2:698089/1‑90 (MQ=255)                                                          cTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAAt                           <  1:718956/90‑1 (MQ=255)                                                           tGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATa                          <  2:831659/90‑1 (MQ=255)                                                           tGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATa                          <  1:587867/90‑1 (MQ=255)                                                              aaCTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGc                       <  2:305015/90‑1 (MQ=255)                                                                  tctcGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCca                   >  1:886733/1‑90 (MQ=255)                                                                            gAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTg                                                 >  1:658398/1‑50 (MQ=255)                                                                            gAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTg                                                 <  2:658398/50‑1 (MQ=255)                                                                            gAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCcatca                <  2:768038/83‑1 (MQ=255)                                                                            gAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCcatca                >  1:768038/1‑83 (MQ=255)                                                                            gAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCcat                  <  1:7449/81‑1 (MQ=255)                                                                            gAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCcat                  >  2:7449/1‑81 (MQ=255)                                                                            gAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCCATCAtaattaa         >  1:28437/1‑90 (MQ=255)                                                                            gAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCCATCAtaattaa         >  1:659812/1‑90 (MQ=255)                                                                            gAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCCATCAtaatta          <  1:587133/89‑1 (MQ=255)                                                                            gAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCCATCAtaatta          >  2:587133/1‑89 (MQ=255)                                                                                 gCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCCATCATAATTAATAGAt    >  1:277271/1‑90 (MQ=255)                                                                                  cTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCCATCATAATTAATAGATa   <  1:837202/90‑1 (MQ=255)                                                                                   tCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCCATCATAATTAATAGATaa  <  2:712408/90‑1 (MQ=255)                                                                                   tCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCCATCATAATTAATAGATaa  <  1:204219/90‑1 (MQ=255)                                                                                       |                                                                                      CAGCGTTTCAGACCATTGCTTATGATAGAATCGATAAGGTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAATACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCCATCATAATTAATAGATAA  >  NZ_CP009273/1838727‑1838898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑