Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,868,213	T→C	F128S (TTC→TCC)	yeaK →	mischarged aminoacyl‑tRNA deacylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,868,213	0	T	C	100.0%	76.0 / NA	22	F128S (TTC→TCC)	yeaK	mischarged aminoacyl‑tRNA deacylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/9);  total (13/9)

TCACAGGCTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGC  >  NZ_CP009273/1868135‑1868299                                                                               |                                                                                       tCACAGGCTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTCCGAACGATTtg                                                                             >  1:708605/1‑90 (MQ=255)          gtgtCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCg                                                                    >  2:692045/1‑90 (MQ=255)               ttCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTc                                                               <  1:356459/90‑1 (MQ=255)                tCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCa                                                              >  1:111612/1‑90 (MQ=255)                    cgcgATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGc                                                          >  1:250195/1‑90 (MQ=255)                    cgcgATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGc                                                          >  2:762753/1‑90 (MQ=255)                     gcgATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCa                                                         >  2:374576/1‑90 (MQ=255)                            ccccTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGc                                                  <  2:708605/90‑1 (MQ=255)                                                      aaaCTGGTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAAcc                        >  2:750690/1‑90 (MQ=255)                                                       aaCTGGTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACcg                       >  1:170177/1‑90 (MQ=255)                                                        aCTGGTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACcgc                      <  2:777103/90‑1 (MQ=255)                                                        aCTGGTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACcgc                      <  2:358676/90‑1 (MQ=255)                                                           ggTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGa                   >  2:69404/1‑90 (MQ=255)                                                            gTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGat                  >  2:383883/1‑90 (MQ=255)                                                            gTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGat                  >  2:446800/1‑90 (MQ=255)                                                                     ccTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATttgcgc         >  1:549443/1‑90 (MQ=255)                                                                      ctctACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATttgcgca        <  1:314349/90‑1 (MQ=255)                                                                       tctACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATttgcgcat       <  1:374576/90‑1 (MQ=255)                                                                           ctctCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATttgcgcattgcg   >  1:139825/1‑90 (MQ=255)                                                                           ctctCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATttgcgcattgcg   <  2:49043/90‑1 (MQ=255)                                                                           ctctCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATttgcgcattgcg   <  1:446800/90‑1 (MQ=255)                                                                           ctctCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATttgcgcattgcg   <  1:416780/90‑1 (MQ=255)                                                                            tctcCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATttgcgcattgcgc  <  1:82081/90‑1 (MQ=255)                                                                               |                                                                                       TCACAGGCTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGC  >  NZ_CP009273/1868135‑1868299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑