Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,358,446	Δ1 bp	coding (414/426 nt)	nudI →	nucleoside triphosphatase NudI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,358,444	0	A	.	100.0%	67.9 / NA	17	coding (412/426 nt)	nudI	nucleoside triphosphatase NudI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (12/5);  total (12/5)

CTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGA  >  NZ_CP009273/2358371‑2358524                                                                          |                                                                                 cTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTG‑AAGGTCTTCTGTAACaa                                                                 >  1:16144/1‑90 (MQ=255) cTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTG‑AAGGTCTTCTGTAACaa                                                                 >  1:650802/1‑90 (MQ=255)  tACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTG‑AAGGTCTTCTGTAACAat                                                                >  1:392811/1‑90 (MQ=255)        tGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTG‑AAGGTCTTCTGt                                                                      <  2:695855/78‑1 (MQ=255)        tGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTG‑AAGGTCTTCTGt                                                                      >  1:695855/1‑78 (MQ=255)         gggTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGATAAACGTTACGTTTG‑AAGGtcttc                                                                         <  1:436172/74‑1 (MQ=255)         gggTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGATAAACGTTACGTTTG‑AAGGtcttc                                                                         >  2:436172/1‑74 (MQ=255)              aaaCCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTG‑AAGGTCTTCTGTAACAAtgatga                                                           >  1:194065/1‑83 (MQ=255)              aaaCCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTG‑AAGGTCTTCTGTAACAAtgatga                                                           <  2:194065/83‑1 (MQ=255)                    gAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTG‑AAGGTCTTCTGTAACAATGATGACAGCCATTACTgg                                              >  1:95990/1‑90 (MQ=255)                          cTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTG‑AAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCa                                        >  2:497006/1‑90 (MQ=255)                            ggTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTG‑AAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGa                                      <  1:28022/90‑1 (MQ=255)                                 aTTATGATTTGAATGTCGCCACCCGAAAAACGTTACGGTTG‑AAGGGCGTCTGAACCAATGATGAAAGCCATTACTGTCTGTCAGATTGaa                                 >  1:436891/1‑90 (MQ=255)                                       aTTTGAATGTCGCCACCCGAAAAACGTTACGTTTG‑AAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTa                           >  2:2882/1‑90 (MQ=255)                                        tttGAATGTCGCCACCCGAAAAACGTTACGTTTG‑AAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAg                          <  2:504620/90‑1 (MQ=255)                                                  gCCACCCGAAAAACGTTACGTTTG‑AAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGa                >  2:617020/1‑90 (MQ=255)                                                                gttacgttTG‑AAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGa  >  2:570470/1‑90 (MQ=255)                                                                          |                                                                                 CTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGA  >  NZ_CP009273/2358371‑2358524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑