Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	340,444	(A)5→4	coding (558/816 nt)	BW25113_RS01680 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	340,440	0	A	.	100.0%	152.0 / NA	36	coding (554/816 nt)	BW25113_RS01680	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (21/15);  total (21/15)

AACAGATTGCAAATGTATTCTTCTTGAATACCATATGCCTTTGCTGAGAATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGGAAAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCATGGATTATTATTACACTT  >  NZ_CP009273/340357‑340524                                                                                    |                                                                                     aaCAGATTGCAAATGTATTCTTCTTGAATACCATATGCCTTTGCTGAGAATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGGaaaatgg                                                                                >  1:636395/1‑87 (MQ=255)      aTTGCAAATGTATTCTTCTTGAATACCATATGCCTTTGCTGAGAATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTcg                                                                          >  1:24466/1‑90 (MQ=255)      aTTGCAAATGTATTCTTCTTGAATACCATATGCCTTTGCTGAGAATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTcg                                                                          >  1:471517/1‑90 (MQ=255)                         tGAATACCATATGCCTTTGCTGAGAATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCg                                                       >  1:387841/1‑90 (MQ=255)                              aCCATATGCCTTTGCTGAGAATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGagac                                                  <  1:752887/90‑1 (MQ=255)                                 atatGCCTTTGCTGAGAATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGagacaga                                               >  2:622048/1‑90 (MQ=255)                                 atatGCCTTTGCTGAGAATTTTCCCTAAAGGGAAAGAGGGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGagacaga                                               >  1:483944/1‑90 (MQ=255)                                    tGCCTTTGCTGAGAATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGAcgc                                            <  1:44865/90‑1 (MQ=255)                                    tGCCTTTGCTGAGAATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGAcgc                                            >  1:663367/1‑90 (MQ=255)                                     gCCTTTGCTGAGAATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGAcgcg                                           <  1:572835/90‑1 (MQ=255)                                     gCCTTTGCTGAGAATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGAcgcg                                           <  1:618611/90‑1 (MQ=255)                                         ttGCTGAGAATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCa                                       >  1:448560/1‑90 (MQ=255)                                         ttGCTGAGAATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCa                                       >  2:497727/1‑90 (MQ=255)                                         ttGCTGAGAATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCa                                       >  2:58039/1‑90 (MQ=255)                                         ttGCTGAGAATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCa                                       >  2:183084/1‑90 (MQ=255)                                         ttGCTGAGAATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCa                                       >  2:74158/1‑90 (MQ=255)                                                gaATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCa                                >  1:482510/1‑90 (MQ=255)                                                       cccTAAAGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCtt                         <  2:755160/90‑1 (MQ=255)                                                             aGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCAt                   <  2:322583/90‑1 (MQ=255)                                                             aGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCAt                   <  2:392727/90‑1 (MQ=255)                                                             aGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCAt                   <  2:159233/90‑1 (MQ=255)                                                             aGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCAt                   <  2:582293/90‑1 (MQ=255)                                                             aGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCAt                   <  2:642648/90‑1 (MQ=255)                                                             aGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCAt                   <  1:497727/90‑1 (MQ=255)                                                             aGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCAt                   <  1:183084/90‑1 (MQ=255)                                                                  aaGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCATGGatt              <  1:622048/90‑1 (MQ=255)                                                                       tgtATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCATGGattattat         >  1:656623/1‑90 (MQ=255)                                                                        gtATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCATGGattattatt        >  2:3159/1‑90 (MQ=255)                                                                        gtATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCATGGattattatt        >  2:246214/1‑90 (MQ=255)                                                                        gtATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCATGGattattatt        >  1:625580/1‑90 (MQ=255)                                                                        gtATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCATGGattattatt        >  1:146444/1‑90 (MQ=255)                                                                        gtATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCATGGattattatt        >  1:1014/1‑90 (MQ=255)                                                                         tATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCATGGAttattatta       >  2:223878/1‑90 (MQ=255)                                                                          aTGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCATGGATTATTATTac      <  2:471517/90‑1 (MQ=255)                                                                              aTGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCAt                   >  2:574636/1‑73 (MQ=255)                                                                              aTGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCAt                   <  1:574636/73‑1 (MQ=255)                                                                              aTGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCATGGATTATTATTACACtt  >  1:100821/1‑90 (MQ=255)                                                                                    |                                                                                     AACAGATTGCAAATGTATTCTTCTTGAATACCATATGCCTTTGCTGAGAATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGGAAAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCATGGATTATTATTACACTT  >  NZ_CP009273/340357‑340524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑