Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 1,223,232 (A)8→7 coding (63/294 nt) BW25113_RS06140 → YcgL domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,223,2250A.100.0% 41.6 / NA 11coding (56/294 nt)BW25113_RS06140YcgL domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (7/4);  total (7/4)

ATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGG  >  NZ_CP009273/1223137‑1223308
                                                                                        |                                                                                   
aTGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGaa                                                                                    >  1:186840/1‑90 (MQ=255)
                       gagTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGTCCTATTTATATGTCGAAAAAAAGACGATTTTTCGCGTGtt                                                            <  2:443870/90‑1 (MQ=255)
                         gTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAGACGATTTTTCGCGTGTTcc                                                          >  2:413980/1‑90 (MQ=255)
                                 aTGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAAc                                                  >  1:436906/1‑90 (MQ=255)
                                                   cGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTc                                <  1:549638/90‑1 (MQ=255)
                                                   cGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTc                                <  2:234755/90‑1 (MQ=255)
                                                                       gACCTATTTATATGTCGAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGAtt            >  1:597444/1‑90 (MQ=255)
                                                                       gACCTATTTATATGTCGAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGAtt            >  1:668125/1‑90 (MQ=255)
                                                                                 tatGTCGAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTc                                >  1:350725/1‑60 (MQ=255)
                                                                                 tatGTCGAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTc                                <  2:350725/60‑1 (MQ=255)
                                                                                 tatGTCGAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTgg  >  1:641439/1‑90 (MQ=255)
                                                                                 tatGTCGAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTgg  >  2:234933/1‑90 (MQ=255)
                                                                                        |                                                                                   
ATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGG  >  NZ_CP009273/1223137‑1223308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: