Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 1,417,788 (C)6→7 coding (69/120 nt) BW25113_RS26130 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,417,7821.C100.0% 45.0 / NA 16G25G (GGA→GGGBW25113_RS26130hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (9/7);  total (9/7)

TCGTCGGTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGCCCAACTTGAA  >  NZ_CP009273/1417706‑1417862
                                                                             |                                                                                
tcgtcgGTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCCGACTgg                                                                      >  2:609777/1‑90 (MQ=255)
                      gTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGatta                                                >  2:350064/1‑90 (MQ=255)
                      gTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGatta                                                >  2:554010/1‑90 (MQ=255)
                            cagtcggtGCGTGAAGCCCCCCCCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTccc                                          <  1:551307/90‑3 (MQ=11)
                                cagtGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTc                                      >  1:192596/1‑90 (MQ=255)
                                cagtGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTc                                      >  2:597389/1‑90 (MQ=255)
                                    gCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGAGTATTTCACCCTCCcgg                                  >  1:306298/1‑90 (MQ=11)
                                                            cGTGGATAATGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGccc          <  1:618001/90‑1 (MQ=255)
                                                            cGTGGATAATGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGccc          <  1:646695/90‑1 (MQ=255)
                                                            cGTGGATAATGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGccc          <  2:192596/90‑1 (MQ=255)
                                                               ggATAATGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGc                >  1:495021/1‑81 (MQ=255)
                                                               ggATAATGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGc                <  2:495021/81‑1 (MQ=255)
                                                                   aaTGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGCCCAACTTGa   >  2:160238/1‑90 (MQ=255)
                                                                    aTGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGa                                 <  1:40374/59‑1 (MQ=14)
                                                                    aTGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGa                                 >  2:40374/1‑59 (MQ=14)
                                                                    aTGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGCCCAACTTGaa  <  1:626769/90‑1 (MQ=255)
                                                                             |                                                                                
TCGTCGGTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGCCCAACTTGAA  >  NZ_CP009273/1417706‑1417862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: