| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 702,597 | 0 | T | G | 56.2% | 9.9 / 15.0 | 16 | V350G (GTG→GGG) | glnS | glutamine‑‑tRNA ligase |
| Reads supporting (aligned to +/- strand): ref base T (2/5); new base G (9/0); total (11/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.80e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACACCATTGAGATGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGC > NZ_CP009273/702510‑702676 | acacCATTGAGATGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATTCGGGGa > 1:384647/1‑90 (MQ=255) ccATTGAGATGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAAc < 2:383780/90‑1 (MQ=255) aTTGAGATGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGGGAAAATg > 2:411530/1‑90 (MQ=255) aTTGAGATGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGGTTTTGATCCGGGGGAACTg > 2:735340/1‑90 (MQ=255) gaTGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGTTTTCGATCCGGGGGAACTGGTTAt > 1:259583/1‑90 (MQ=255) gaTGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGTTATTGATCCGGGGGAAATgggttt > 1:692902/1‑86 (MQ=255) ggCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGGTTTCGATCCGGGGAAACTGGTTATTGa > 1:110424/1‑90 (MQ=255) aTCCGTGAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGAACCGGTGAAAATGGTTATTGAAAACCATCAGggcgaaggg > 2:65320/1‑89 (MQ=255) tctcAACGAAAATGCGCCGCGCGCAATGGCGGGTATCGATCCGGTGAAACTTGGTATCGAAAAATATCAAGGGGGAGGCGGAAAGGTTAc > 2:748398/1‑90 (MQ=255) tcAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGGGAAACTGGTTATCGAAAAATATCAGGGGGAAGGGGAAAAGGTTACCa > 2:318554/1‑90 (MQ=255) cAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGGGGAACTGGGTATCGAAAAATATCAGGGCGAAGGGGAAAAGGTTACCAt > 1:770416/1‑90 (MQ=255) aTGCGCCGCGCGCAATGGCGGTTTTTGATCCGGGGAAACTGGTTATCGAAAAATATCAAGGGGGAGGCGAAATGGGTACCAAGCCGAAcc > 1:596003/1‑90 (MQ=255) cGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGaaa < 1:735340/90‑1 (MQ=255) aTCGATCCGGTGTAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGc < 1:70542/90‑1 (MQ=255) aTCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGc < 1:386534/90‑1 (MQ=255) aTCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGc < 2:110424/90‑1 (MQ=255) | ACACCATTGAGATGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGC > NZ_CP009273/702510‑702676 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |