| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,154,068 | 0 | T | G | 72.7% | ‑0.1 / 11.9 | 22 | G248G (GGT→GGG) | ptsG | PTS glucose transporter subunit IIBC |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (16/0); total (17/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.28e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.33e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTG‑TTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCT > NZ_CP009273/1153981‑1154140 | gATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGGaa > 2:367843/1‑90 (MQ=255)gATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGGaa > 2:32003/1‑90 (MQ=255) tGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAAc < 1:715006/90‑1 (MQ=255) aTTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCt < 2:715930/90‑1 (MQ=255) acacCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTcc < 1:185051/90‑1 (MQ=255) ccAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGGAAACTGTCTGGGGGCTTCCTG‑t > 2:221466/1‑90 (MQ=255) aCGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGGAAACTGTCTGGGGGCTTTCTG‑TTCa > 2:199130/1‑90 (MQ=255) aCGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGGGACCCCAATGCGGGGGAAATGTCTTGTGGGTTCCTG‑tttt > 1:686642/1‑88 (MQ=255) aCGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGCGACCCCACTGCGGGGAAACTGTCTGGGGGGCTCCTG‑TTCa > 2:568390/1‑90 (MQ=255) gcagGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGATTGAGGGGAAACTTTTTGGGGGTTTCCCT‑TTTAAAAtt > 2:185616/1‑89 (MQ=255) gcagGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGAACGCGGGGAAACTGGCTGGTGGGTTCCTG‑TTCAAAATg > 2:123160/1‑90 (MQ=255) gcagGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGGGGCCCGACCGCGGGGAAAATGGCTGGTGGCTTCCTGTTTCaaaag > 1:639705/1‑89 (MQ=255) gcagGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGGGACCCCACTGCGGGGAAACTGTCTGGGGGGTTCCTG‑TTCAAAATg > 1:624009/1‑90 (MQ=255) ggtcaggtTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGGAAACTGTCTGGTGGCTTCCTG‑TTCAAAATGTAc > 2:450949/1‑90 (MQ=255) tCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTG‑TTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGc < 1:367843/90‑1 (MQ=255) gcgTTATATGGCGGGGGACCCGACTGCGGGGAAACTGTCTGGGGGCTTCCTG‑TTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTAt > 2:459090/1‑90 (MQ=255) tGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTG‑TTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCAct < 2:105606/90‑1 (MQ=255) tGGCGGGTGACCCGACTGCGGGGAAACTGTCTGGTGGCTTCCTG‑TTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCAct > 2:378227/1‑90 (MQ=255) gCGGGTGACCCGACTGCGGGTAAACTGTCTGGGGGGTTTCTG‑TTCAAAATGTACGGGCTGCCAGCTGGCGCAATTGCCATCTGGCActct > 1:93868/1‑90 (MQ=255) gCGGGTGACCCGACTGCGGGGAAACTGTCTGGTGGCTTCCTG‑TTCAAAATGGACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCActct > 1:279366/1‑90 (MQ=255) cGGGCGACCCGACTGCGGGGAAACTGTCTGGGGGCTTCCTG‑TTTAAAATTTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGGACTCTg > 1:588184/1‑90 (MQ=255) ggTGACCCGACTGCGGGGAAACTGTCTGGTGGCTTCCTG‑TTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCt > 2:534184/1‑90 (MQ=255) | GATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTG‑TTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCT > NZ_CP009273/1153981‑1154140 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |