Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,598,1800TG57.1% 8.8 / 19.7 23V302G (GTG→GGG) lsrCautoinducer 2 ABC transporter permease LsrC
Reads supporting (aligned to +/- strand):  ref base T (2/7);  new base G (12/0);  total (16/7)
Fisher's exact test for biased strand distribution p-value = 3.10e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.98e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAAC  >  NZ_CP009273/1598092‑1598269
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aCGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGgtg                                                                                          <  2:162563/90‑1 (MQ=255)
               gTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGGGTTTGATGGGCGCCTg                                                                           >  2:300263/1‑90 (MQ=255)
               gTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGGGCTGGGGTTTTTTGGACGcccg                                                                           >  1:380370/1‑88 (MQ=255)
                    ggTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTTATGGTCGCCTTCGttt                                                                      >  1:747755/1‑89 (MQ=255)
                    ggTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTtg                                                                      <  2:106300/90‑1 (MQ=255)
                    ggTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGGGGTTTATGGGCGCCTGCGGtg                                                                      >  1:774431/1‑90 (MQ=255)
                    ggTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGGGGTGGGGGTTGGTTGACGCCTGCGttt                                                                      >  1:217316/1‑89 (MQ=255)
                         tGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGGGTTTGATGGACGCCTGCGTTGTgcgc                                                                 >  2:565456/1‑90 (MQ=255)
                         tGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGGGGTTGGTGGGCGCCCGCGTTGTgcgc                                                                 >  1:284977/1‑90 (MQ=255)
                         tGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGGGCTGGGGTTTGATGGACGCCTGCGTTGTgcgc                                                                 >  2:293143/1‑90 (MQ=255)
                            tGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGGGCTGGCGTTTGTTGGGGCCCTGCGTTGGGCGCTgg                                                              >  2:503674/1‑90 (MQ=255)
                            tGCGCATGCCGGCATGGGGGAATGATTTTATCGCGGGGCTGGTTCGGCTGGCGGTGCTGGTGTTTGATGGGGGCCTGGGTTGTGGGGTgg                                                              >  1:649509/1‑90 (MQ=255)
                              cgcATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGaa                                                            <  2:538729/90‑1 (MQ=255)
                               gcATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGGGTTTGATGGGCGGCTGCGTTTGGGGCTGGTAc                                                           >  1:341140/1‑90 (MQ=255)
                                  ttCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGGGCTGGGGTTTGATGGACGCCTGCGTTGTGCGCTGGAACgga                                                        >  1:554743/1‑88 (MQ=255)
                                    ccGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGGGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAAt                                                      >  2:323853/1‑90 (MQ=255)
                                          tggtggAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTAcgg                                                <  2:112486/90‑1 (MQ=255)
                                            gtggAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGGGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGGAATCTAcggcg                                              >  2:644432/1‑90 (MQ=255)
                                                      tttATCGCGGGTCTGGTTCTGCTGGCGGGGCTGGGGTTTTATGGGCGCCTGCGGTGTGCGCTGGAACGGAATCTACGGCGGGaaaaaaaa                                    >  1:36180/1‑87 (MQ=255)
                                                          tCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGccc                                <  1:630083/90‑1 (MQ=255)
                                                              gggTCTGGTTCTGCTGGCGGGGCTGGCGTTTGGTGGTCGCCTGGGTTGTGCGCGGGAAGGTAATCTACGGCGGGAAAAATATTGCCGcgt                            >  1:150963/1‑88 (MQ=255)
                                                                              gCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCa            <  2:247134/90‑1 (MQ=255)
                                                                               cGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCAt           <  2:486631/90‑1 (MQ=255)
                                                                               cGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCAt           <  1:620700/90‑1 (MQ=255)
                                                                                        tgtTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAAc  <  2:129597/90‑1 (MQ=255)
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ACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAAC  >  NZ_CP009273/1598092‑1598269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: